Laser photochemistry of provitamin D

The most important results

  • Breakdown of photostationary state approximation has been revealed under monochromatic laser irradiation at the wave-lengths > 300nm.

    • I.P.Terenetskaya, S.I.Gundorov, V.I.Kravchenko, E.B.Berik "Nanosecond laser photolysis of provitamin D", Sov.J.Quantum Electronics, 1988, Vol.18, pp.1323-1327.

    N.A.Bogoslovsky, I.K.Berik, S.I.Gundorov, I.P.Terenetskaya "Characteristics of laser photolysis of provitamin D", High Energy Chem., 1989, Vol.23, pp.218-222.

    I.P.Terenetskaya, S.I.Gundorov, E.B.Berik "Characteristics of photolysis of provitamin D by long-wavelength radiation", Sov.J.Quantum Electron., 1991, Vol.21, pp.472-474.

    S.I.Gundorov, V.A.Davydenko, I.P.Terenetskaya, O.I.Yuschuk "Study of the kinetics and quantum efficiencies in the photochemistry of provitamin D using a tunable laser", Sov.J.Quantum Electron., 1991, Vol.21, pp.339-343.

    I.P.Terenetskaya "Lasers in Vitamin D synthesis", Romanian Journal of Biophysics, 1995, Vol.5, №4, pp.263-277

    I.P.Terenetskaya "Limitations of the photostationary approximation in provitamin D photochemistry", Theor. Experim. Chem., 2008, Vol.44, №5, pp.286-289.

  • New intensity effects were observed under picosecond laser irradiation, the physical principles of the reciprocity law violation were established and adequate mathematical model was proposed with due regard for non-equilibrium of previtamin D conformers.

    • I.P.Terenetskaya, Yu.A.Repeyev "Provitamin D photoisomerization kinetics upon picosecond laser irradiation: the role of previtamin conformational non-equilibrium", in "Laser Applications in Life Sciences", Part Two: Lasers in Biophysics and Biomedicine, SPIE Proc., Vol.1403, 1990, pp.500-503.

    A.A.Serikov, I.P.Terenetskaya, "The influence of radiation intensity on the pathways of phototransformations of conformationally mobile molecules", High Energy Chem., 1994, Vol.28, №3, pp.257-262.

    I.P.Terenetskaya, Yu.A.Repeyev "Features of laser photosynthesis of previtamin D under high intensity picosecond irradiation", High Energy Chemistry, 1996, Vol.30, №5, pp.402-406.

    Yu.A.Repeyev, I.P.Terenetskaya "Laser photosynthesis of previtamin D: new effects under high-intensity picosecond irradiation", Sov.J.Quantum Electron., 1996, Vol.23, №8, pp.765-768.

  • The ways of purposeful impact on the conformational distribution of previtamin D and its photoreaction channels were specified through the characteristics of laser UV radiation and interaction with microenvironment in different reaction media.

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Practically important results

  • On the basis of carried out analysis of destructive influence of the irreversible channel on the photoproduct yield, practical recommendations were given which have allowed essentially to raise previtamin D yield in industrial production.

    • I.P.Terenetskaya, O.N.Galkin, N.A.Bogoslovsky "Irreversible reactions in the photochemistry of provitamin D under optically dense layer conditions", Pharm.Chemistry J., 1993, Vol.27, №4, pp.282-288.

    I.P.Terenetskaya, Y.A.Samojlov, N.A.Bogoslovsky, L.N.Vysotsky, F.I.Luknitsky "Analysis of the two-stage irradiation of provitamin D taking into account the irreversible photoconversions of previtamin", Pharm.Chem.J., 1993, Vol.27, №11, pp7-803.

    I.P.Terenetskaya, N.A.Bogoslovsky, L.N.Vysotsky, F.I.Luknitsky "Routes to optimization of previtamin D photosyntesis using irradiation by a sunlamp", Pharm.Chem.J., 1994, Vol.28, №8, pp.589-596.

  • The express-method of spectrophotometric concentration analysis of the multicomponent photoisomer mixture has been developed taking into account its irreversible photodegradation. As a result, in situ monitoring of biologically active solar UV radiation became possible using an in vitro model of previtamin D photosynthesis.

Greek UV spectra concentration kinetics
Transformation of the provitamin D absorption spectrum and corresponding concentration kinetics (symbols – experimental data, lines – calculated data).

I.P.Terenetskaya "Provitamin D photoisomerization as possible UVB monitor: kinetic study using tunable dye laser", SPIE Proceedings of the Int. Conf. "Biomedical Optics'94", January 22-28, 1994, Los Angeles, USA, Vol.2134B “Ultraviolet Radiation Hazards”, pp.135-140.

I.Terenetskaya "Vitamin D Biodosimeter: monitoring of antirachitic activity of solar UVB radiation", EC Air Pollution Research Report 71 "Biological UV dosimetry, a tool for assessing the impact of UV radiation on health and ecosystems", 1999, pp.158-168.

A.R.Webb, I.P.Terenetskaya "Combined study of antirachitic solar UVB radiation by spectroradiometer and "Vitamin D" biodosimeter", In: "Biologic Effects of Light 1998”: Proceedings of a symposium, Basel, Switzerland, Nov.1-3, 1998 / Editors, Michael F. Holick and Ernst G. Jung, Kluver Acad. Publishers, 1999, pp.153-155.

Irina P.Terenetskaya "The new method of UV dosimetry based on an in vitro model of previtamin D photosynthesis”, Internet Virtual Conference “Protection Against the Hazards of UVR", Jan.18-Feb.5, 1999.

O.N.Galkin, I.P. Terenetskaya "Vitamin D" biodosimeter: basic characteristics and prospect applications", J.Photochem.Photobiol. B:Biology, 1999, Vol.53, №1, pp.12-19.

I.Terenetskaya "Spectral Monitoring of Biologically Active Solar UVB Radiation Using an In Vitro Model of Vitamin D Synthesis", Talanta, 2000, Vol.53, №1, pp.195-203.

I. Bolsee, A. R. Webb, D. Gillotay, B. Dorschel, P. Knuschke, A. Krins, and I. Terenetskaya "Laboratory facilities and recommendations for the characterization of biological ultraviolet dosimeters", Applied Optics, 2000, Vol.39, №16, pp.2813-2822.

Irina Terenetskaya "A possibility of ozone depletion monitoring in conditions of opaque atmosphere using ‘D-dosimeter’, In: Ultraviolet Ground and Space -based measurements, Models and Effects. Eds Slusser JR, Herman JR and Gao W., SPIE Proc., 2002, Vol.4482, pp.305-312.

Irina Terenetskaya "Duality of solar UV-B radiation and relevant dosimetry: vitamin D synthesis versus skin erytema", (#4896-25), In: Protection Against the Hazards of UVR, Hangzhou, China, 4896, edited by W. Gao, G. Shi, K. Shibasaki, and J. Slusser, SPIE Proc., 2003, Vol.4896, pp.144-150.

I. Terenetskaya "Solar UV-B dosimetry in situ with ‘D-dosimeter’: effect of ozone depletion on the vitamin D synthetic capacity of sunlight", Agriculture and Forest Meteorology, 2003, Vol.120, pp.45-50.

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