|
Position: Deputy Head of Department of physics of biological systems Institution attended: Institute of Physics, NAS Ukraine Scientific interests: Biophysics, Nucleic acids, Solid State Physics, Surface Solids, experimental physics-FTIR, SEIRA, UV, Raman, SERS, NMR spectroscopy, Langmuir-Blodgett technique, AFM microscopy, computational chemistry, nanotechnology, single walled carbon nanotubes, C60 and the complexes. |
Lecture title: Surface enhanced spectroscopy for study of biomolecules and cells
Lecture abstract: Small amount of the substance under study that the searchers should work with in biology, medicine, pharmacology, art, etc. imposes some restrictions on the use of traditional techniques and makes necessary specific methods. For this reason, for analysis of the samples of extremely small amount, we proposed a new approach for registering the spectra on the basis of the effect of enhancement. The enhancement of optical process by a factor 102..106 near rough surface of metal (Au, Ag, Cu, etc.) is known already for thirty years, for both optical transitions in adsorbed molecules (Raman scattering (RS) of light, luminescence, infra red (IR) absorption and the processes which do not depend on the presence of molecules on the metal surface (for example, second harmonics generation). The enhancement of infrared (IR) absorption by rough metallic surface is named as SEIRA (surface enhanced infra red absorption) and enhancement of RS is named as SERS (Surface enhanced Raman Scattering). The effect lies in an essential increase of the intensity of transition (for example, effective cross-section increases by factor 105..106 for RS, 10-104 for IR absorption, 10-102 for luminescence) or efficiency of the process near metal surface. Here we demonstrate and discuss different applications of SEIRA, SERS, enhanced fluorescent spectroscopy and imaging in nanotechnology, medicine and biology as well new possible substrates as carbon nanostructures for it.