Nanobiotechnology for health-care

Influence of the silver nanoparticles treatment 
on reproductive function under experimental systemic autoimmune disorder in mice

Sribna V.O., Stupchuk M.S., Kaleinikova O.M., 
Blashkiv T.V., Voznesenskaya T.Yu.
Bogomoletz Instytute of Physiology National Academy of Sciences of Ukraine, Kyiv, Ukraine
E-mail: valia-z@ukr.net

Nowadays, biomedical nanotechnology and nano medicine develops rapidly in the search for new drugs. Among them drugs based on silver nanoparticles (AgNPs) occupy the leading position. 
The aim of this work was to study the influence of the treatment of the substance of AgNPs, Resveratrol and Nicotinamide (activator and inhibitor of the Sirtuins, respectively) on oocyte meiotic maturation, damage of genome`s integrity (DNA single-strand breaks) of the follicular cells surrounding the oocytes, pre- and post-implantational embryonic mortality rates in mice. 
Experimental systemic autoimmune disorder in mice was achieved by immunization of white laboratory mice of the first generation with a kidney antigen suspension derived from a parent. Nanoparticles (AgNPs) - 30 nm (concentration: 8 mg/ml for metal, shape: spherical, color: brown) synthesized at the Ovcharenko Institute of Biocolloidal Chemistry NAS of Ukraine in accordance with the original protocol (by chemical condensation). The treatment was carried out the following way: kidney antigen suspension - intraperitoneal three times 1 time per day; the procedure was repeated in 3 weeks, one time intraperitoneally with the same dose (10 mkL of suspension per 10 grams of body weight of the animal). AgNPs – intravenous three times: 1 time per day for 1 hour before immunization of animals with suspension of kidney antigen; as well as in 3 weeks once with the same dose (2 mg/kg).
We were obtained the new data about the influence of the treatment of the substance of AgNPs, Resveratrol and Nicotinamide on oocyte meiotic maturation, damage of genome`s integrity (DNA single-strand breaks) of the follicular cells surrounding the oocytes, pre- and post-implantational embryonic mortality rates in mice.
The obtained results confirm the working hypothesis about the antioxidant properties of the experimental substance of the AgNPs, as well as the possibility of participation of the Sirtuins in the implementation of the reproductive function.