Nanochemistry and biotechnology

Comparison of the possibility of using a calixarene based sensor and a two enzymes based biosensor for the arginine analysis 
I.S. Kucherenko1, O.O. Soldatkin1,2, S.V. Marchenko1, O.V. Soldatkina2, V.O. Prilipko3, M.A. Kuibida3, S.O. Cherenok4, O.S. Prynova4, O.M.Sylenko4, O.I. Kalchenko4, V.I. Kalchenko4, S.V. Dzyadevych1,2
1 Institute of Molecular Biology and Genetics of NAS of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine. E-mail: alex_sold@yahoo.com 

2 Institute of High Technologies, Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01003 Kyiv, Ukraine.

3 National Aviation University, Kosmonavta Komarova Str., 1, 03058, Kyiv, Ukraine

4Institute of Organic Chemistry of NAS of Ukraine, Murmanska Str., 5, 02660, Kyiv, Ukraine

L-arginine plays a key role in maintaining vascular homeostasis and endothelial function, it is involved in the pathogenesis of many cardiovascular diseases, including hypertension, coronary heart disease, peripheral and coronary dysfunction, and atheroma. Determination of L-arginine concentration in serum allows diagnosis and studying the clinical course of argininamia, broncho-obstructive syndrome, pulmonary tuberculosis, stomach cancer and colorectal cancer.
To date, a number of methods are known for determination of L-arginine concentration, in particular, fluorometry, spectrophotometry, ion-exchange chromatography, polarography, capillary and zone electrophoresis. However, most of the known methods have disadvantages, such as low selectivity and sensitivity, high-cost and complex equipment. Additionally, the measurement procedure is time-consuming and requires highly qualified personnel. Today, electrochemical sensors are increasingly considered as a successful alternative to classical method.
In this work, two types of sensor for determination of arginine were created. Interdigitated gold electrodes deposited on the ceramic support were used as conductometric transdusers. As a sensitive elements was used two enzyme or calixarene based membranes. The analytical characteristics of both sensors were analyzed and compared. The use perspective of both developed sensors for analysis of arginine in biological fluids was proven.