Nanobiotechnology for health-care

Vanadium nanoparticles as reproductive correctors
Karpenko N.A.1, Belkina I.O.1, Koreneva E.M.1, Chistyakova E.E.1, Smolenko N.P.1, Kustova S.P1, Boiko M.A.1, Klochkov V.K.2
1 SI “V. Danilevsky Institute for Endocrine Pathology Problems of the NAMS of Ukraine” Alchevsky, 10, Kharkiv, Ukraine. 
E-mail: inna-bel@mail.ru
2 SI “Institute for Scintillation Materials, National Academy of Sciences of Ukraine”, Nauky ave, 60, Kharkiv, Ukraine.

Increasing of the frequency of male reproductive disorders is a background of searching of innovative hypofertility medicines. Chemical compounds in the nanoparticle form have new chemical and physical properties and toxicity which depend on particles form, size, composition, environment, regimen of introduction and etc. Vanadium nanoparticles became more attractive due to their different biological effects including influence on the reproductive system. Therefore, the aim of the researching was to determine the impact of nanoparticles of Gadolinium Orthovanadate (NPs GdVO4) on the rat’s male reproductive functioning under the condition of the chronic using of pure nanoparticles and their pharmaceutical composition.
It has been shown that chronic using of NPs GdVO4 has positive impact on the reproductive function of rat’s males with reproductive disorders which had been induced in the neonatal period. Differences of the biological activity between hydrosol of GdVO4 and their pharmaceutical composition have been determined. These differences may be explained by the influence of the auxiliary substances on nanoparticles's properties in the pharmaceutical composition. After measuring of average hydrodynamic diameters of nanoparticles complexes, it has been determined that hydrodynamic diameters of nanoparticles in hydrosol and pharmaceutical composition has important differences, in some cases more than 100nm. It may affect the solution stability and permeability of NPs GdVO4 through cell's membrane.
Obtained results demonstrate that GdVO4 nanoparticles may be taken to consideration as potential medicine for treatment male reproductive disorders. Additional researches for pharmaceutical composition development are necessary.