Nanobiotechnology for health-care

Influence of carbon nanotubes and its derivatives on biochemical parameters, cellular blood composition in vivo
O.M.Perepelytsina1, A.P. Ugnivenko1, D.P. Burlaka1, O.V. Yastrebova1, 
O.N.Bakalinska2, M.V.Sydorenko1
1 Department for biotechnical problems of diagnostic IPCC, Nauky str.,42/1, Kiev 03028, Ukraine. E-mail: olenaquail@gmail.com
2Chuiko Institute of Surface Chemistry NAS of Ukraine, 17 General Naumov str., Kiev 03164, Ukraine. 

	One of the most striking and promising applications of carbon nanotubes is medicine. The aim of present study was to analyze influence of oxygenized carbon nanotubes (CNTox) and CNT which were functionalized by Doxorubicin (CNT-Dox) on organism of mouse in long-term period. 
	It was investigated affect of the CNTox, CNT-DOX on the state of hepatic enzymatic system, the protein metabolism and cell blood composition of the experimental animals. As parameters of hepatic enzymes were determined Alanine aminotransferase (ALT), Aspartate aminotransferase (AST) and Alkaline phosphatase (ALP); as markers of protein metabolism were analyzed level of albumin (Al) and total protein (Tp) in blood serum. The possibility of inflammatory processes and systemic effects of CNTox, CNT-Dox was investigated by assay of blood cells composition.
	Long-term parenteral administration of CNTox, CNT-Dox, and pure doxorubicin was realized during 30 days. As result, CNTox administration didn`t leaded to statistically significant changes in AST, ALT and ALP levels but slightly increased the level of total protein in the blood of experimental animals. CNTox didn’t demonstrated statistically significant influence on mouse blood cell composition. And opposite, administration of CNT-Dox and Dox had toxicity influence on organism and induced a chronic hepatic damage. Symptoms of hepatitis had more manifestation after Dox treatment than CNT-Dox. Assay of the blood cell formula of the experimental animals after the administration of  CNT-Dox and Dox demonstrated the well-known manifestations of DOX hematological toxicity: anemia, thrombopenia, neutropenia. A decrease of total toxic effect of CNT-Dox, compared to the effect of pure Doxorubicin, on the metabolic processes of the experimental animal was demonstrated. Deviations in the cell blood composition indicated a general toxic effect of CNT-Dox, but it was more moderate compare with administration of pure doxorubicin. From the data obtained, we conclude that it is possible to use CNT as carrier for controlled drugs release in certain conditions, for example in the stomach, intestines.