Nanochemistry and biotechnology

Comparison of the influent of silver ions and silver nanoparticles on the growth of Fusarium culmorum sp.
Kasprowicz M.J.1, Szymocha A.1
1 Department of Physics, Institute of Soil Science and Agrophysics. University of Agriculture in Krakow. Al.Mickiewicza 21, 31-120 Kraków, Poland. 
E-mail: marek.kasprowicz@ur.krakow.pl

The aim of the present experiment was to investigate the influence of silver nanoparticles and silver ions on Fusarium culmorum (W.G. Smith) Sacc. (FC) spores. The silver nanoparticles were produced by the high-voltage arc discharge method [1-2]. 
The vegetative mycelia growth on potato dextrose agar (PDA) medium after 1, 24 and 72 hour contact of FC spores with 25 ppm water solution of silver nanoparticles or/and silver ions. A significant reduction in mycelial growth was observed for spores incubated with silver nanoparticles after 1 and 72 hours. Silver ions (silver nitrate water solutions) exhibited a fungistatic effect for conidia after 1, 24 and 72h. 
Second parameter tested in the experiment was the formation of spores after the mycelia were cultured. The incubation of FC spores with silver ions and silver ions and silver nanoparticles mixture greatly reduced the number of spores formed by mycelia.
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1.	Kasprowicz M.J., Gorczyca A., Janas P.  Production of silver nanoparticles using high voltage arc discharge method // Current Nanosciences.-2016.-12, N 6.-P. 747-753.
2.	Kasprowicz M.J.., Kozioł M., Gorczyca A. The effect of silver nanoparticles on phytopathogenic spores of Fusarium culmorum // Can J Microbiol.-2010.-56, N 3.-P. 247-253.