Nanobiotechnology for health-care
Urotropine-containing antibacterial nanocomposite materials and their physico-chemical properties
Skurikhina O.1, Kozakevych R.2, Liubatska O.1, Murlanova T.1, Vakuliuk P.1, Lupatsii M.1, Furtat I.1, Tertykh V.2, Golub A.1
1National University of Kyiv-Mohyla Academy, 2 Skovoroda Str., Kyiv, 04070, Ukraine. Email: tanmurlanova@gmail.com

2Chuiko Institute of Surface Chemistry of the NAS of Ukraine
17 General Naumov Str., 03164, Kyiv, Ukraine

The wound cleansing from a pathogenic microbial medium and the effective removal of the wound exudate are the main factors should be taken into consideration for the effective treatment of wound infections. The use of pyrogenic silica as a carrier of drugs provides not only delivery of antimicrobial agents, but also ensures effective removal of the wound exudates and wound cleansing [1].
Nanocomposites based on highly dispersed pyrogenic silica, urotropine, and combined nanocomposites containing both ornidazole and urotropine with different concentrations of active components in nanocomposites were obtained in the research. The concentration of each antibacterial component was 2% and 4% for one component and for mixed nanocomposites, respectively. The presence of ornidazole and urotropine on the surface of modified nanocomposites was confirmed by the method of IR spectrometry. The kinetics of desorption of active components from the nanocomposite surface was investigated by the method of adsorption spectrophotometry (for ornidazole-containing nanocomposites) and titrimetry (for urotropine-containing) for all samples for 12 hours. Analyzing the kinetics of desorption for 2AU (A=aerosil, U=urotropine) and 4AU samples, it can be concluded that a larger part of urotropine is desorbed into the solution during the first hour. The release of antimicrobial substances from composites with a combined composition was two times slower than for samples with pure urotropine.
The antimicrobial activity of the obtained nanocomposites was investigated for Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli strains. It was shown that the antimicrobial effect was greater while using nanocomposites based on ornidazole and urotropine, than the effect of nanocomposites with only one active compound, due to the phenomenon of synergy.

1.	Murlanova T., Kotsiuda S., Vakuluk P., Golub A. Nanocomposites based on ornidazole and pyrogenic silica for the treatment of complicated wounds // Scientific notes of NaUKMA. Natural Sciences. – 2017. – 197, p. 53-57.