Nanochemistry and biotechnology

Cadmium and essential metal nanoparticles influence on the oxidative stress parameters of lettuce plants
Khomenko I.M., PysmennaY.M., Kosyk O.I., Taran N.Yu.
Plant Biology Department, Educational and Scientific Center “Institute of Biology and Medicine” of Taras Shevchenko National University of Kyiv. Volodymyrska St., 64/13, Kyiv-01601, Ukraine. 
E-mail: i.m.homenko@gmail.com 
Nanoparticles of biologically active metals, as of today, are widely used in agriculture, since they are considered environmentally safe promoters of plant resistance to stress factors of diverse nature [1]. The aim of the present study was to determine the influence of cadmium ions and essential metal nanoparticles on the oxidative stress parameters of two lettuce (Lactuca sativa L.) varieties with different level of anthocyanin pigments. Samples of the green Lolo and red Lolo Ross variety were selected on the vegetative maturity phase during 14 days of 0,1 μmol Cd2+-exposure (31st-44th days after pre-sowing treatment with the mix of metal nanoparticles – Cu, Zn, Fe, Mn). The investigated parameters were presented by changes in hydroperoxide (HP) and malonic dialdehyde content (MDA), and guaiacol-peroxidase (GP) activity with the use of spectrophotometry methods [2].
 The slight reduction in the content of HP in contrast of significant MDA-content increase (70%) was determined in the Lolo variety under the action of cadmium and co-influence. The combined effect resulted in an increase of GP activity on the first day with a further decrease during exposure. In red Lolo Ross variety slightly increase of HP was fixed under the cadmium effect as opposed to the combined treatment (75%-increment). Both treatment variants were manifested the maximal growth of MDA content on the first day (twice the value of control) with substantive decline throughout the exposition (down to 21-26%). The GP-activity growing was more intensively fixed in red Lolo Ross variety in all treatment variants. Thus, all treatment variants were manifested as stress-factors in both lettuce plants.

1.	Juárez-Maldonado A., González-Morales S., Cabrera-De la Fuente M., Medrano-Macнas J., Benavides-Mendoza A. Nanometals as Promoters of Nutraceutical Quality in Crop Plants // Impact of Nanoscience in the Food Industry.-2018.-P. 277-310. 
2.	Taran N., Batsmanova L., Kovalenko M., Okanenko O. Impact of Metal Nanoform Colloidal Solution on the Adaptive Potential of Plants // Nanoscale Res Lett.-2016.-P. 11-89.