Nanochemistry and biotechnology 

Influence of bentonite particles of various sizes on growth activity and synthesis of compounds of phenolic nature of Bacillus subtilis IMV B-7023
Skorochod І.О., Roy А.О., Kurdish І.К.
Department of Microbiological Processes on Solid Surfaces, Zabolotny 
Institute of Microbiology and Virology, National Academy of Sciences of
Ukraine, 154, Acad. Zabolotny St., Kyiv, MSP D03680, Ukraine
E-mail: aphalina.77@gmail.com

Bentonite is a mineral of montmorillonites group, which has almost all properties of natural nanoscale particles [1]. This nanomaterial is widely used in various fields of industry. In particular, on the basis of bentonite and two strains of bacteria Azotobacter vinelandii IMV B-7076 and Bacillus subtilis IMV B-7023 created a biological preparation for plant growth Azogran [2]. It should be borne in mind that particles of bentonite can have both nanoscale and micro size, which affects their properties. Learning a concept for the interaction of nanoscale and micro particles with living organisms is an extremely important issue. The aim of the work was to investigate the influence of different size bentonite particles on growth activity and synthesis of compounds of phenolic nature (Ph-OH) B. subtilis IMV B-7023.
Dependence of growth activity of bacillus and its synthesis of Ph-OH on the size of bentonite particles was revealed. The most pronounced negative effect on both parameters had bentonite nanoparticles, in comparison with effect of bentonite micro particles (the size is more than 100 nm). Accordingly, after 48 hours of cultivation of B. subtilis IMV B-7023 in glucose-mineral medium with sodium glycerophosphate and with bentonite nanoparticles, the amount of viable cells decreased by 5.6 times and the amount of Ph-OH in their culture medium was decreased by 17.6% relative to control in which the mineral was not introduced.

1.	Hochella M. F., Lower S. K., Maurice P. A. Nanominerals, mineral nanoparticles and earth systems // Science – 2008. –319, №5870. – P. 1631–1635.
2.	PATENT of Ukraine № 54923А. Strain of bacteria Bacillus subtilis for bacterial fertilizer obtaining for plant-growing / І. К. Kurdish, A. O. Roy. - Published in 2003, bulletin № 3 (in Ukraine).