Nanocomposites and nanomaterials

Study of physical interactions of amphiphilic copolyester of fluorescein with albumin in aqueous dispersions

Yakoviv M.V.1, Nosova N.G.1, Samaryk V.Y.1, Varvarenko S.M.1, Pasetto P.2
1 Department of organic chemistry, Lviv Polytechnic National University, 12, Stepan Bandera str., Lviv-79013, Ukraine. 
E-mail: ferensmariia@gmail.com
2 Institut des Molécules et Matériaux du Mans, UMR CNRS 6283, Le Mans Université, Avenue Olivier Messiaen 72085 Le Mans Cedex 9, France.

Development of novel materials with useful properties for medical applications remains an important trend in the area of polymer chemistry. New delivery systems for therapeutic drugs are composed of macromolecules able to self-assembly in the aquatic medium and the formation of disperse systems that provide prolonged protection of drugs and active substances from the hydrolytic action of the environment and the immune attack of the body. Researchers continuously study the behavior of such systems in the organism.
We have obtained new amphiphilic copolyester based on the derivatives of amino acids and polyether diols. These copolyesters also contain a given number of fluorescein fragments. Thus, synthesized polymers are extremely promising for practical applications as they combine the possibilities of drug delivery and diagnostic systems.
We have investigated the sorption of bovine serum albumin (BSA) on particles in copolymer dispersion and obtained the dependences of the aggregate stability of the dispersions on the composition and structure of copolymers. It has been established that the majority of synthesized copolyester possess effects associated with the additional stabilization of dispersed phase particles by albumin. Such stabilization leads to the decrease in the average particle diameter in dispersions. 
The copolyester in the complex with sorbed albumin does not lose the ability to redistribute in the model medium water/n-octanol. However, it tends to increase the holding of the copolyester in the aqueous phase. However, this effect does not lead to a significant deviation from the range of values that indicate the possibility of macromolecules with sorbed albumin distribute in both water and lipophilic phases. The ability of synthesized copolyester to transition between water and lipophilic phase has provided an effective basis for drug delivery systems with fluorescein fragments that provide the possibility to develop diagnostic tools.