Nanochemistry and biotechnology

Dependence of growth and superoxide dismutase activity of Bacillus subtilis IMV V-7023 from the content of nanoparticles of natural minerals and ions of mangan and iron.
Кurdish I.K., Chobotarov A.Yu., Gritsay R.V.
Department of Microbiological Processes on Solid Surfaces, Zabolotny Institute of Microbiology and Virology of the NAS of Ukraine, 154, Akad. Zabolotnogo Str. Kyiv, 03143, Ukraine
E-mail: ivan.kurdish2016@gmail.com

The application of microbial preparations in crop production is the actual trend in correcting biotic processes in agroecosystems and a significant increase in their productivity. The interaction of our selected strains of phosphate-mobilizing microorganisms of Bacillus subtilis IMV В-7023 and nitrogen-fixing bacteria of Azotobacter vinelandii IMV В-7076 with particles of clay mineral bentonite was used to elaborate a highly efficient complex bacterial preparation Azogran for crop production purposes. Once Azogran preparation is used in crop production, the bacteria in its composition may interact with nanoparticles of natural minerals (bentonite, saponite, etc.), which are important components of the soil. The impact of these particles on the growth and SOD activity of this strain of bacilli is yet to be studied. Thus, aim of this work was to determine the impact of nanoparticles of natural minerals and ions of mangan and iron, which are included in the active center of the SOD a bacteria, on the growth and superoxide dismutase activity of B. subtilis IMV В-7023 – a component of complex bacterial preparation Azogran.
It was established that the cultivation of B. subtilis IMV В-7023 for 24 h in the Spizizen medium with the introduction of 1 g/l of saponite or bentonite particles was accompanied with the increase in their growth activity. Adding to such a medium 0.1 - 0.5 mM Mn2+ ions stimulated the growth activity of bacteria. The growth of bacilli in a medium with similar concentrations of ferrous cations without nanoparticles had a little effect on the growth of B. subtilis. However, adding 1 g / l of saponite to such medium showed a significant increase in the growth activity of bacteria, while nanoparticles bentonite almost did not affect this index. The cultivation of B. subtilis in the medium, containing Mn2+ ions, stimulated their superoxide dismutase activity insignificantly. When particles of saponite and especially bentonite were added to this medium, the indices of this activity increased considerably. The introduction of ferrum ions to the medium stimulated SOD activity of this strain, amounting to maximal indices at the content of 1.5 mM Fe2+.