Thematic area         Nanobiotechnology for health-care
	Metal nanoprisms for theranostics

Estrela-Llopis V. R.1, Lytvyn P. M. 2, Fesenko O. M3., Shton I. O4.

1 F. D. Ovcharenko  Institute of Biocolloidal Chemistry NAS of Ukraine. 42, Akademika Vernadsky blvd., Kyiv, 03142, Ukraine, victorio.estrela@gmail.com
2 V.Lashkaryov Institute of Semiconductor Physics NAS Ukraine. 41 ave. Nauki, 03028, Kyiv.
3  Institute of Physics of the NAS of Ukraine. ave. Nauki 46 Kyiv 03028
4 RE Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine. Vasylkivska str 45, Kyiv-03022.

The scientific direction theranostics unites the areas of diagnostics and therapy. The term arose recently in connection with the effective use of gold and silver nanoparticles in these two areas, primarily in the field of oncology. In contrast to quasispherical particles, nano-prisms of gold and silver show a more intense and broadband plasmon resonance, which determines their advantage in both bio-detection based on LSPR and when using such nanoprisms in photodynamic therapy of cancer. 
This report presents the results of two “green” synthesis of micro- nanoprisms (thickness - 30-40 nm, linear size - up to 5 μm) and nanoprism (thickness - 10-15 nm, linear size - 40-80 nm) of gold and silver. The morphology and crystal structure of such nanoprisms are investigated by scanning probe microscopy, optical, electron microscopy with Rt-microanalysis, FT-IR and UV-VIS spectroscopy. Polysaccharides (PS) of microalgae are used here as a reducing and stabilizing agent. Such high molecular weight anionic polysaccharides possess “stealth” and “smart” properties, they determine the selective endocytosis of bioconjugates nanoprism-PS in a cancer cell. The toxicity of nanogold action on cancer cells is associated with the catalytic properties of such particles, the toxicity of a nanosilver - with the action of dissolved silver anions, the toxicity of polysaccharides is assoiated primarily with their immunostimulating properties.
The dose-dependent and selective cytotoxic effect of such nanoprisms on the blood cancer cells (in comparison with healthy lymphocytes), ovarian cancer, Ehrlich carcinoma, and Lewis lung carcinoma (photodynamic therapy) is shown.
	Using the method of micro-Raman spectroscopy, it was shown that the intensity of the spectral modes of protein, polysaccharides and pyridostigmine (analog of the chemical warfare agent - sarin) adsorbed on gold nanoprisms increases by several orders of magnitude in comparison with the Raman spectra of these individual substances. This result demonstrates the effectiveness of using such nanoprisms to increase the sensitivity of biodetection as a result of the localized surface plasmon resonance.