Preparetion and characterization of silver nanoparticles (AgNPs) and their applications as biological agent

H. Ünver1, B. Boyacioglu2, M. Yıldız3, Neslihan Demir4, Nuray Yıldırım5, Ayhan Elmalı6
1Department of Physics, Faculty of Sciences, University of Ankara, TR-06100 Besevler-Ankara, Turkey. 
E-mail: email.of. corresponding_Huseyin.Unver@ankara.edu.tr
2Vocational School of Health Services, Ankara University, TR-06290 Kecioren-Ankara, Turkey
3Department of Chemistry, Faculty of Sciences and Arts, Çanakkale Onsekiz Mart University, TR-17100 Çanakkale, Turkey.
4Department of Biology, Faculty of Arts and Sciences, Çanakkale Onsekiz Mart University, 17100 Çanakkale, Turkey
5Vocational School of Health Services, Çanakkale Onsekiz Mart University, 17100 Çanakkale, Turkey
6Department of Physics Engineering, Faculty of Engineering, Ankara University, TR-06100, Beşevler , Turkey

The antibacterial activity of Ag-GQDs has been evaluated and compared to that of bare GQDs and commercial silver nanoparticles (Ag-NPs) against both Gram-negative and Gram-positive bacteria [1,2]. The effective antibacterial concentrations of Ag-GQDs have been not affecting the viability of human and animal cells, which suggest that they have biocompatible and ecofriendly. According to these results also suggest that Ag-GQDs nanocomposites may be applied as antibacterial material, such as in: antibiotics, coating of surgical instrumentation, wound dressing textiles, disinfectants, antiseptic and detergents [1]. Here, AgNPs are prepared by using GQDs and silver nitrate and hydrothermal reaction as starting materials and synthesis method. AgNPs/GQDs Nanocomposites are characterized by FT-IR spectroscopy, UV-VIS spectroscopy, Transmission electronmicroscopy (TEM), thermogravimetric analysis (TGA) and Dynamic light scattering (DLS) methods. The antimicrobial activity of the nanoparticles was investigated for its minimum inhibitory concentration (MIC) to bacteria and yeast cultures. The interactions between the GQDs and DNA were investigated using UV-Visible spectroscopy and agarose gel electrophoresis. 

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