Nanochemistry and biotechnology

Effect of Cu/salicylic acid nanocolloid on plant-fungal pathogen interactions

Lopatko S. K. 1, Trach V. V. 2, Lopatko K. G. 3, Voloschuk N. M. 3, Pysmennaia Y. N.1, ,Smirnov O. E. 1

1 Taras Shevchenko National University of Kyiv, Educational and Scientific Center "Institute of Biology and Medicine", Department of Plant Physiology and Ecology, st. Vladimir, 64/13, Kyiv, Ukraine 
E-mail: lopatkos15@gmail.com
2 Institute of Plant Physiology and Genetics of NAS of Ukraine, Vasil’kovskaja 31/17, 03022, Kiev, Ukraine
3 National University of Life and Environmental Sciences of Ukraine, Gerojev Oboroni Str. 15, 03041 Kiev, Ukraine

Increasing the resistance of phytopathogens to fungicides, is a serious problem of the present.  Accumulation of the actual data on this problem is driving force for the development of new fungicidal solutions. One of the promising way to decide this problem is implementation nanoparticles. The nanoparticles are recived by elecro – spark disarge. This method which is based on the new physical effect in the industry of high energy concentration. This nanoparticles (with the size from 10 – 100 nm and less) are characterized mechanical, catalytic, adsorption properties because their properties related with laws of quantum mechanics. Also, we obtained a solution of nanoparticles based on salicylic acid. This acid is phytohormone and increase stress tolerance of plants. Nowadays, metal nanoparticles are used un the agrarian undustry as micro- and macro- elements of plant nutrition. Some nanoparticles that are capable to inhibition of pathogenic fungi are part of fungicides. Copper compounds were used as a means of protecting plants from infectious diseases. Сooper - containing fungicides are characterized by contact-prophylactic and protective action. But their fungicidal action is more effective against propagation spores of pathogens than spread of mycelial fungus. 
	The nanoscale copper used in this study was produced at the material science department of National University of Life and Environmental Sciences of the Ukraine. Characteristics of the particles were as follows. Average particle size of 40-50 nm., purity – 99,9%.Salicylic acid, can serve as a regulator of transport of organic substances in floes, gravitation and other physiological processes. All these data allow us to consider salicylic acid as promising for the practical application of the compound to protect plants from majority of stres factors.
	Based on these data, we conducted a series of experiments with the aim to compare the action of nanoparticles in colloidal form and in the form of copper salicylate. We identified their influence on the cultures of some phytopathogens of cereal cultures common in the continental climate. Also, it was explored influence on wheat affected by Fusarium verticillioides.