Preparation, caharacterization and biological application of palladium nanoparticles (PdNPs) based on nitrogen-doped porous carbon particles (GQDs)

Mustafa Yıldız1, Nurettin Şahiner1, Nuray Yıldırım2, Neslihan Demir3, Hüseyin Ünver4, Bahadır Boyacıoğlu5, Ayhan Elmalı6
1 Department of Chemistry, Faculty of Arts and Sciences, Çanakkale Onsekiz Mart University, Çanakkale, Turkey
E-mail: email.of. corresponding_myildiz@comu.edu.tr
2Vocational School of Health Services, Çanakkale Onsekiz Mart University, Turkey
3 Department of Biology, Faculty of Arts and Sciences, Çanakkale Onsekiz Mart University, Çanakkale, Turkey
4 Department of Physics, Faculty of Science,  Ankara University, Ankara, Turkey
5 Vocational School of Health Services, Ankara University, Ankara, Turkey
6Department of Physics Engineering, Faculty of Engineering, Ankara University, TR-06100, Beşevler , Turkey

Recently, many studies were conducted on graphene quantum dots nanomaterials [1-3]. In this study, palladium nanoparticles (PdNPs) were synthesized using nitrogen-doped porous carbon nanomaterials (GQDs) as reducing reagent and stabilizer [1]. Nanocomposites consisting of palladium nanoparticle and graphene quantum dots (GQDs/PdNPs) were characterized by UV-Vis, FT-IR spectroscopy, transmission electronmicroscopy (TEM) and thermogravimetric analysis (TGA). UV-Vis spectroscopy studies of the interactions between the PdNPs and GQDs with calf thymus DNA (CT-DNA) showed that the compound interacts with CT-DNA via electrostatic binding.  The DNA cleavage activity of the PdNPs and GQDs was studied by agarose gel electrophoresis method. pBR322 DNA  in Tris-HCl buffer treated with the compound at 37 C for 3 h. DNA cleavage study showed that the PdNPs and GQDs cleaved DNA without any external agents.
Support from Çanakkale Onsekiz Mart University, The Scientific Research Commission (ÇOMÜ-FBA: 2018-1291) is greatly acknowledged.

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