Nanochemistry and biotechnology

MAGNETIC RESONANСE NANOTHERANOSTICS FOR MALIGNANT TUMORS

Orel V.E. 1, Shevchenko A.D.2, Golovko T.S.1, Ganich O.V.1, Rihalsky O.Yu. 1, Orel I.V.1

1 National Cancer Institute, 33/43 Lomonosov St., 03022, Kyiv, Ukraine
2 G. V. Kurdyumov Institute for Metal Physics, Natl. Acad. of Sci. of Ukraine, 36, Academician Vernadsky Blvd., 03680, Kiev-04142, Ukraine
E-mail: admit@imp.kiev.ua

The purpose of the current work is to experimentally prove the possible use of the magnetic resonance effect for malignant tumor nanotheranostics.
Materials and methods. The study was carried out on 40 C57Bl / 6 mice bearing Lewis lung carcinoma. Animals were divided into 4 groups: 1 - control (without treatment); 2 - doxorubicin (DOXO) (Pfizer) administration; 3 - administration of a mechano-magneto-chemically synthesized (MMCS) nanocomplex comprising ferromagnetic commercial nanoparticles <50 nm (Sigma-Aldrich) and paramagnetic DOXO; 4 - administration of the MMCS nanocomplex with following whole-body electromagnetic irradiation (EI) produced by a magnetic resonance tomography (MRT) system Intera 1.5T (Philips Medical Systems) in animals. 
Results and discussion. The administration of officinal DОХО or nanocomplex alone without ER resulted in the equal minimum antitumor effect. However, nanotherapy combined with whole-body ER by MRT initiated the maximum antitumor effect in laboratory animals. The average number of lung metastases per animal in groups 3 and 4 was determined to be less than in groups 1 and 2 on 25th day after tumor transplantation. The obtained results were confirmed by MRT studies of animals with Lewis lung carcinoma. Based on a previously conducted investigation of malignant tumor cells in vitro, it can now be considered that the enhanced antitumor effect was due to reactive oxygen and nitrogen species generation under the conditions of the magnetic resonance effect initiated by the MMCS nanocomplex in malignant tumors. Conclusions. Therefore, the technology of magnetic resonance nanotheranostics could improve the antitumor effect of chemotherapeutic agents and control the treatment effectiveness which opens new possibilities for future clinical use of nanotechnology in individually optimized treatment protocols for cancer patients with disseminated tumors.