Nanobiotechnology for health-care

Prostate protective and sperm modulating effects of the combined nanoparticles based on rare-earth metals oxides
Korenieva Ye.M.1, Karpenko N.A.1, Smolienko N.P.1, Chistyakova E.E.1, Belkina I.O.1, Kustova S.P1, Boiko M.O.1, Klochkov V.K.2
1 SI “V. Danilevsky Institute for Endocrine Pathology Problems of the NAMS of Ukraine”. Alchevsky, 10, Kharkiv, Ukraine.
E-mail: reproduk@ipep.com.ua
2 SI “Institute for Scintillation Materials, National Academy of Sciences of Ukraine”, Nauky ave, 60, Kharkiv, Ukraine.

The importance of the prostate protectors developing is caused by spreading of men's reproductive system inflammatory diseases, among which the chronic prostatitis takes the first place. 
The aim: to assess the effectiveness of the nanoparticles (NP) composition based on rare-earth elements oxides (ORE) and silver nanoparticles used for treatment reproductive system disturbances caused by experimental chronic prostatitis.
Materials and methods: the prostatitis in rats has been induced by cryogenic traumatizing of the prostate gland's ventral part. Investigation has been carried out on two groups of animals (rats of 200 – 250 body weight). The first group received hydrosol which contained NP GdVO4:Eu3+ (group chronic prostatitis +NP+ORE); the second group received hydrosol which contained NP GdVO4:Eu3+ and Ag (group chronic prostatitis+ NP ORE+ Ag). NP have been administrated in a dose of 0,3 mg/kg. Control group – false operated rats. 
Results: rectal using of NP ORE gel reduced inflammation (with leucocytes number), normalized the blood level of steady metabolites of nitric oxide cycle and did not decrease rat's body weight, prostate gland weight and reproductive organs weight relative to prostatitis. Experimental group NP ORE+ Ag has demonstrated spermogram quality improvement and normalizing of body weight gaining. The increasing of absolute and relative epididymides weight and decreasing of inflammation response have been determined. However, the statistic tendency to increasing NOx blood level has fallen further. 
Conclusion: the prostate protective and sperm modulating activities of combined nanoparticles based on rare-earth elements oxides have been determined.