Nanochemistry and biotechnology
«Green» synthesis of silver nanoparticles using Pleurotus eryngii  (DC.) Quel.
Pirko Ya.V1., Danylenko I.A.1., Krupodorova T.A.1, Kvasko A.Yu.1, Klymchuk D.O.2 , Smertenko P.S.3, Blume Y.B.1

1.Institute of Food Biotechnology and Genomics, NAS of Ukraine, Osypovskoho Street, 2a, Kyiv, 04123, Ukraine.
E-mail:yarvp1@gmail.com 

2.M.G. Kholodny Institute of Botany, NAS of Ukraine. Tereshchenkivska Street, 2, Kyiv, 01004, Ukraine.
3.V.Ye. Lashkaryov  Institute of Semiconductor Physics, NAS of Ukraine, avenue Nauki, 41, Kyiv, 03028, Ukraine.

Silver nanoparticles represent one of the most popular nanomaterials with unique properties. They can be used in biological and biomedical research as well as pharmaceutical and many other applications [1, 2]. Most of the chemical and physical methods of silver nanoparticles synthesis are expensive and they are not ecologically friendly. Some of them use the toxic precursors. The different approaches for “green” synthesis (using microorganisms, fungi or plant tissue) were proposed as possible environmentally safe methods which are alternatives to existing chemical and physical methods of nanoparticle synthesis [3, 4]. There is already published information about the application Pleurotus ostreatus from Basidiomycetes fungi for the synthesis of nanoparticles [5]. Therefore the purpose of this study is to develop a new eco-friendly method for «green» synthesis of silver nanoparticles by Pleurotus eryngii (DC.) Quel. and investigate their properties. 
 Silver nanoparticles were synthesised using mycelium of the P. eryngii. Cultivation of fungi was carried out in 100 mL Erlenmeyer flask with 50 mL of Glucose Yeast Peptone liquid medium under 28˚С for 10 days. On the tenth day the surface mycelium was aseptically separated from cultural liquid and washed repeatedly with sterile distilled water (not less then 5 times) to eliminate the residual culture medium. 0,1М solution of AgNO3 was poured into the flasks with fungal mycelium in order to produce silver nanoparticles. Obtained brown solution was filtered through Millipore filter (USA) (pore diameter of 100 nm) and used then for optical and structural studies. Absorption spectra of obtained nanoparticles were measured using a spectrophotometer Specord UV-VIS. Characterization of morphology of nanoparticles was carried out using electron microscope JEOL JEM-2100F (Japan). 
It was observed that synthesized nanoparticles have spherical shape and have a size from 5 to 40 nm. Absorption maximum corresponded to 430 nm. Also several types of tissues from natural and artificial fibers, which will be modified with silver nanoparticles are investigated to determine their antibacterial properties. It is proposed to modify the tissues with additional binding agents including polyaniline.

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