Nanocomposites and nanomaterials
Synthesis and Spectroscopic Characterization of Hybrid Nanosystems “D-g-PAA/AuNanoparicle/Chlorin e6/Dox”

A. Naumenko *, N. Kutsevol, V. Chumachenko, Iu. Harahuts
Taras Shevchenko National University of Kyiv, Volodymyrs’ka St., 
01601, Kyiv, Ukraine 
E-mail: a_naumenko@univ.kiev.ua
In modern medicine, the development of systems for the selective delivery of drugs directly into the affected cells is relevant, without affecting the healthy cells. In recent years, the possibility of using nanocomposites based on polymers as targeted delivery of photosensitizers (PS) for photodynamic antitumor therapy (PDT) is actively explored. Nanocomposites containing PS have a number of advantages over the initial photosensitizing drugs, since they prevent the aggregation of PS molecules, which leads to a decrease in its activity. In addition, nanocomposites based on polymers can be additionally loaded with various drugs, which in turn enhance the effect of treatment.
Polymer matrices on the base of Dextran core Polyacrylamide (neutral and anion forms) as well as systems consisting of incorporated in a polymer matrix of gold nanoparticles and photosensitizer molecules or doxorubicin (2), triple systems “D-g-PAA/AuNPs/PS or Dox”(3), and, finally, the systems”D-g-PAA /AuNPs/PS/Dox" (4) have been sequentially investigated by methods of optical spectroscopy (spectrophotometry, photoluminescence, luminescence excitation spectra) . The measurements of spectra at various concentrations were carried out to determine the optimal composition of nanosystems. 
It was found that in the presence of a photosensitizer or doxorubicin, an insignificant aggregation process in the system occurs as a result of the aggregation of the polymeric component due to the change in the hydrophilic-hydrophobic balance of the polymer matrix. This confirms the fact that Chlorine e6 and Doxorubicin interact with Polymer matrices.Aggregation processes are significantly enhanced in the four-component nanosystem D-g-PAA /AuNPs/Chlorine e6 /Doxorubicin, but the size of the gold nanoparticles does not change.
Nanocomposite synthesized in anionic branched polymer matrix D-g-PAA /AuNPs/Chlorine e6 is recommended for in vitro testing on cell cultures using photodynamic therapy, while the composite D-g-PAA /AuNPs/Dox - for testing for chemotherapy.