Nanochemistry and biotechnology

Domains of molybdopterin-dependent oxidoreductase of Natrialba aegyptia
Venger O.1, Venger A.2
1 Plant Breeding and Genetics Institute – National Center of Seed and Cultivar Investigation, Ovidiopol’ska doroga Str., 3, Odesa-65036, Ukraine. emerald-olga@ukr.net
2 Department of microbiology, virology and immunology, Odessa National Medical University, Valychovski Lane, 2, Odesa-65000, Ukraine.

In general, molybdopterin-dependent oxidoreductase (MDO) binds molybdopterin as a cofactor. Overall MDO includes several forms of nitrate reductase (Nap, Nas, NarG), formate dehydrogenase-H and -N, thiosulfate reductase, dimethylsulfoxide reductase (DMSOR), formylmethanofuran dehydrogenase, arsenite oxidase. In many bacterial and archaeal species [1], MDO is in the form of a dinucleotide, with two molybdopterin dinucleotide units per molybdenum. Depending on the protein and organism, these proteins can function as monomers, heterodimers, or heterotrimers. The mentioned above included in the MopB superfamily is the eukaryotic/eubacterial protein domain family of the 75-kDa subunit/Nad11/NuoG (second domain) of respiratory complex 1/NADH-quinone oxidoreductase which is postulated to have lost an ancestral formate dehydrogenase activity and only vestigial sequence evidence remains of a molybdopterin binding site [1]. The aim of this work was to detect the domains of the archaea Natrialba aegyptia which have been yet undescribed by the means of DELTA-blast program [2]. As result, such domains as Nitrate-R-NapA, BisC, PRK13532, Molybdopterin MopB_CT_Fdh-Nap-like, Molydop_binding and Molybdop_Fe4S4 were detected.

1.	Marchler-Bauer A., Bo Y., Han L., He J., Lanczycki C., Lu S., Chitsaz F., Derbyshire M.K, Geer R., Gonzales N., Gwadz M., Hurwitz D., Lu F., Marchler G., Song J., Thanki N., Wang Z., Yamashita R., Zhang D., Zheng C., Geer L., Bryant S. CDD/SPARCLE: functional classification of proteins via subfamily domain architectures. // Nucleic Acids Res. -2017.-45, N 1.-P 200-203.
2.	https://blast.ncbi.nlm.nih.gov