Nanochemistry and biotechnology
Caco-2 cell studies of p-tert-butlycalix [4] arene nanofibers with different functional groups
Ozcan F.
 Department of Chemistry, Faculty of Science & Advanced Technology Research and Application Center, Selcuk University, 42075 Konya, Turkey
fatihzcan06@gmail.com
Supramolecules are considered as cyclic macrocyclic compounds with at least nine members and at least three heteroatoms. Examples include; crown ethers, cryptants, cyclophanes, natural cyclodextrins and calixarenes. The fact that Calixarenes is a ring structure, can be easily derivatized and they are good carriers for cation, anion, neutral molecules and biological materials because they can create large molecular voids. Due to these features, they have a very wide application area [1,2]. p-tert-butylcalix[4]arene molecules bearing Nitrile (-CN), Amine (-NH2), Carboxylic acid (-COOH) and Alkyl Bromide (-Br) were synthesized under appropriate reaction conditions, and the synthesized p-tert-butylcalix[4]aren derivatives were formed by electrospinning method. Structures of these synthesized macrocyclic compounds were clarified by spectroscopic techniques (FTIR, 1 H-NMR), nanofiber surface analysis SEM technique. Samples of p-tert-butylcalix[4]arene nanofibers bearing Nitril (-CN), Amine (-NH2), Carboxylic acid (-COOH) and Alkyl Bromide (-Br), counted as Caco-2 cells 15x104, After incubation, the nanofibers were withdrawn and transferred to new 24-well plates and 500 μL of DMEM and 250 μL of XTT (2,3-Bis- (2-Methoxy-4-Nitro-5-Sulfophenyl) -2H-Tetrazolium- 5-Carboxanilide) (Biological Industries) will be added. It is hoped that the calixarenes will lead to different studies on living things. The reason for this prediction, various surface-modified Calixarene of nanofibers, can provide a better platform that supports the adhesion and proliferation of colon cells. Consequently, Calixarene nanofibers may be a suitable approach for biomedical and tissue engineering applications[3].
1.  Ozcan F., Bayrakci  M., Ertul S. Synthesis and Preparation of Novel   Magnetite NanoparticlesContaining Calix[4]arenes With Different Chelating Group Towards Uranium Anions // Journal of Macromolecular Science-2015.-Part A 52.-P. 599–608. 
     2. Ozcan F., Bayrakci M., Ertul S. Synthesis and characterization of novel         nanofiber based calixarene and its binding efficiency towards chromium and   uranium ions // J Incl. Phenom Macrocycl Chem.-2016.- 85.-P.49–58.
     3. Dhir, S., Morrow, K. J., Rhinehart, R. R., Wiesner, T.  Dynamic optimization of     hybridoma growth in a fed‐batch bioreactor // Biotechnology and Bioengineering- 2000.-67.-P 197-205.