Nanotechnology and nanomaterials

Green synthesis and characterization of silver nanoparticles using Grape pomace aqueous extract
Vorobyova V.I.1, Vasyliev G. S.1, Skiba M.I.2, Nahirniak S.V.1, Kuzmenko O.M.1, Khrokalo L.A.1
1 National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”. Prosp. Peremohy, 37, Kyiv-03056, Ukraine.
E-mail: g.vasyliev@kpi.ua
2 Ukrainian State University of Chemical Technology. 
Dzerzhinskogo str. 1/3, Dnipro-49000, Ukraine.

Green-nanotechnology is a newly emerging stream in nanotechnology which is primarily focused on the method and technique of nanomaterial green synthesis [1]. Amongst all the biological materials used in the nanoparticle synthesis, the plant extract mediated nanoparticle synthesis is quicker and the synthesized nanoparticles possess higher stability [1-2]. The current research is aimed at developing eco-friendly method for the synthesis of AgNPs using Grape pomace aqueous extract (GPAE) and evaluation of their anti-bacterial activity. The GPAE contains both stabilizing and reducing agents required for this preparation. To enhance the extraction of active compounds, the extraction process was intensified by the application of ultrasound. Phytochemical composition of GPAE was analyzed by GC–MS. A new method for the determination of reducing activity of the plant extract prior to synthesis based on the cyclic voltammetry on the glassy carbon electrode was tested. The highest reducing ability was found after 48 hours after extraction. 
The AgNPs were characterized by zeta-potential measurements, UV–Vis spectroscopy (absorption peak at 432 nm) and scanning electron microscopy.

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