Nanobiotechnology for health-care
The influence of star-like dextran-polyacrylamide polymers on the lymph nodes cells 

Sribna V.O.1, Harahuts Yu.I.2, Kaleinikova O.M.1,
Blashkiv T.V.1, Kutsevol N.V.2
¹ Bogomoletz Institute of Physiology, NAS of Ukraine, Kyiv, Ukraine
E-mail: valia-z@ukr.net
² Taras Shevchenko National University of Kyiv, Kyiv, Ukraine

It has already been shown that polymers with a dextran core and grafted polyacrylamide chains dextran-polyacrylamide (D-PAA) are effective in photodynamic chemotherapy. Therefore, studies with the use of animals in which the effect of the treatment of the polymeric matrices D-PAA and D-PAA(PE) (dextran-polyacrylamide(polyelectrolyte)) on mammalian cells will be determined. The purpose of the work is to investigate the influence of the treatment of star-like dextran-polyacrylamide polymers on the cells of the lymph nodes in mice. 
Experiments have been conducted on pubescent female Albino mice. There were employed a method of double fluorescent vital assay and methods of statistical data processing. Were used star-like dextran-polyacrylamide polymers (70-80 nm): 0.39 mg/kg, 3.90 mg/kg D-PAA  and 0.39 mg/kg, 3.90 mg/kg           D-PAA(PE). Control animals were treated with physiological solution.	Thus, it has been established that single intravenous treatment of: 
1) 3.90 mg/kg D-PAA decreases the number of living cells of the lymph nodes and increases the number of such cells with morphological signs of apoptosis and necrosis compared with values ​​ in group with 0.39 mg/kg D-PAA single intravenous treatment, respectively, 82,33±2,04% (p<0.001, n=6), 12,42±1,32% (p<0.01, n=6), 5,25±0,82% (p<0.01, n=6) and 86,75±1,08%, 9,67±1,17%, 3,58±0,80%. 
2) 3.90 mg/kg D-PAA(PE) - decreases the number of living cells of the lymph nodes and increases the number of such cells with morphological signs of apoptosis, compared with values ​​ in group with 0.39 mg/kg D-PAA(PE) single intravenous treatment, respectively, 80,58±2,40% (p<0.01, n=6), 14,17±1,54% (p<0.01, n=6), and 83,58±1,11%, 11,33±1,33%. 
In this work we have obtained new data about the influence of star-like            D-PAA and D-PAA(PE) on the lymph nodes cells in mammals, which had not previously been investigated.