Nanochemistry and biotechnology
Advantages and limitations of application of noble metal-based nanozymes of oxidoreductase activity
Pietrzak M.1,2, Ivanova P.1,2, Mizerska M.1, Zbonikowski R.1, Drozd M.1,2, Malinowska E.1,2
1 Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, Poland
E-mail: mariusz@ch.pw.edu.pl
2 Center for Advanced Materials and Technologie, Warsaw University of Technology, Poleczki 19, 02-822 Warsaw, Poland

Due to several limitations such as: high cost of isolation of enzymes, complications in the effective covalent conjugation of enzymes with bioreceptors and their susceptibility to inactivation, the intensification of scientific efforts focused on a replacement of natural enzymes with their inorganic mimetics has been observed in recent years [1]. Various nanoparticles such as metal- or carbon-based ones exhibit interesting properties such as catalytic activity, which allow to use them instead of enzymes (especially oxidoreductases) for some applications. They can be applied in a role of receptor layers of sensors or as labels when conjugated to proteins or nucleic acids, thus allowing the determination of many important analytes. However, the completely different nature of nanoparticles in comparison to native enzymes poses some unexpected limitations in their use. This talk will cover the most interesting aspects of applications of noble metal-based nanoparticles as peroxidase, catalase, superoxide dismutase, polyphenol oxidase etc. It will answer the question, if nanozymes (nanoparticles exhibiting enzyme-like activity) can be treated as more universal than enzymes and give some valuable tips an solutions for the use of nanoparticles as nanozymes.

1.	Wu J., Wang X., Wang Q. et al. Nanomaterials with enzyme-like characteristics (nanozymes): Next-generation artificial enzymes (II)// Chem Soc Rev.-2019.-61.-P. 1004-1076.