Nanobiotechnology for health-care

Hydroxyapate/Сhitosan/Fullerene C60 composites as system for prolonged drug release
1Kumeda M.A., 1Sukhodub L.B., 1Sukhodub L.F. 2Prylutskyy Yu.I
1Sumy State University, Rimsky-Korsakov,2, Sumy-40007, Ukraine.
2 Taras Shevchenko National University of Kyiv, Kyiv, Ukraine 
E-mail: mariyakumeda@gmail.com

Carbon nanoparticles (CNPs) are of great interest especially for tissue engineering, since they contribute to the formation of porous structures similar to the extracellular matrix, where the cells form tissues and organs [1]. CNPs have significant potential for application in bone tissue regeneration, stimulating the differentiation of induced cells in vivo [2] In the last decade, great attention was paid to drug delivery and drug release systems based on nanoparticles of different origins [3]. Among them, there are CNPs due to their ability to cross cell membranes. Surface properties, hydrophilicity, size and shapes make it possible to use CNPs as a vehicle to transport drugs into cells [4]. 
In this work bioactive composite material in the form of beads, based on hydroxyapatite (HA), Chitosan (CS) and C60 fullerene (C60), was synthesized by the "wet chemistry" method under influence of microwave irradiation. Beads obtained by cross-linking of CS macromolecules with sodium tripolyphosphate form the 3D-matrix, which contains immobilized particles of HA and C60. Аccording to the HPLC studies, C60-containing composites provide a prolonged release of hydrophobic Anaesthesinum (used as a model drug) up to 18 days. Changing the C60 content in samples allows adjusting the drug release time. The Anaesthesinum release is close to the zero-order kinetic release profile in accordance with the calculated value of the correlation coefficient (r=0.99).

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