Nanobiotechnology for health-care

Co-doped CdS quantum dots and their bionanocomplex with protein: interaction and bioimaging properties
Stolyarchuk I.D.1, Wojnarowska-Nowak R.2, Nowak S.2, Romerowicz-Misielak M.2, Kuzyk O.V.1, Dan’kiv O.O.1, Stolyarchuk A.I.1
1 Department of Physics Drohobych Ivan Franko State Padagogical University, 24 I. Franko Str., 82100 Drohobych, Ukraine. 
E-mail: i.stolyarchuk@dspu.edu.ua
2 2Center for Microelectronics and Nanotechnology, University of Rzeszow, 16C Rejtana Str., 35-959 Rzeszow, Poland
3 Department of Animal Physiology and Reproduction, Institute of Applied Biotechnology and Basic Sciences, University of Rzeszow, Werynia 502, 36100 Kolbuszowa, Poland
Semiconductor nanoparticles or quantum dots (QDs) constitute significant materials for many аrеas of nano- and biotechnology. One of the main decisive parameters for the use of QDs in bionanotechnology is the functionalization of their surface with appropriate (bio)molecules to ensure the stability of colloidal solutions in combination with effective labeling of the corresponding biological objects. It is the combination of nanoparticles with selected biological or chemical ligands, such as proteins, antibodies or DNA sequences etc., that leads to the creation of highly functional bionanomaterials (bionanocomplexes), which opens up the broadest prospects for their use in bio- and nanomedicine [1].
In the present work, we report on spectroscopy studies of interactions between colloidal CdS:Co diluted magnetic semiconductor QDs and model proteins type of human serum albumin (HSA). To probe this interaction, in addition to conventional UV-Vis absorption and photoluminescence spectroscopic methods we have used micro-Raman spectroscopy and technique of magneto-optical Faraday rotation, which is important for case of nanoparticles with magnetic impurities. It was proved that, in all cases, the fluorescence quenching of HSA by the QDs depends on the size, temperature and electron-deformation interactions. The low toxicity as well as high stability of the QDs - HSA bioconjugates in the case of their using as bio-imaging probes for the 143b osteosarcoma cells has been demonstrated. 

1.	A. Soldado, L. Cid Barrio, M. Diaz-Gonzalez, A. de la Escosura - Muniz, J. M. Costa-Fernandez Advances in quantum dots as diagnostic tools // Adv Clin Chem. 2022; 107:1-40 doi:10.1016/bs.acc.2021.07.001