“Nanobiotechnology for health-care”

Electrophoretic studies of proteins of erythrocytes membrane under influence of silver nanoparticles
Batyuk L.V.1, Kizilova N.N.2
1 Department of Medical and Biological Physics and Medical Information Science, Kharkiv National Medical University, 4 Nauky Ave., Kharkiv, 61022, Ukraine.
E-mail: liliya-batyuk@ukr.net
2 V. N. Karazin Kharkiv National University, Kharkiv, Ukraine

The application of silver nanoparticles (AgNPs) for treatments against different diseases is increasingly innovative and effective [1]. The venous blood erythrocytes of 20 patients with lung cancer and 20 healthy donors were used as the object of the study. The control group consisted of 20 practically healthy donors of the same age. The effect of AgNP preparations (diameter d~35 nm) on RBCs membranes in a physiological solution containing 65.25 ∙ 106 cells per 1 ml of the reaction mixture was analyzed. Membranes of erythrocytes were obtained by the method of J. Dodge [2]. Isolation and separation of the membrane proteins of erythrocytes by one-dimensional disc-electrophoresis in a 10% polyacrylamide gel containing 1% sodium dodecyl sulfate was carried out according to the well-known Laemmli method [3].The resulting gels were scanned on a densitometer. Calculations of the percentage content of individual protein fractions (before and after its introduction of AgNP preparations in suspension in vitro) were performed based on the analysis of densitograms. Statistical processing was carried out using the t-criteria Students. Comparative analysis of the protein spectrum of erythrocyte membranes of blood of patients with cancer before and after AgNP preparations added revealed significant changes in the content of spectrin, ankyrin, and bands of proteins 2 and 3; in proteins of band of fractions 4.1, 4.2 and 4.9, protein of band 6 and protein of band 8.
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2.	Batyuk L., Kizilova N., Muraveinik O. Assessment of Protein Fractions of RBCs in Stroke under Influence of Nanodiamonds in vitro// NAP-2018, 2018 IEEE 8th International Conference on Nanomaterials: Applications & Properties.-2018.- 4.-P.4-9.
3.	Laemmli U. K. Cleavage of structural proteins during the assembly of the head // T4. Nature.-1970.- 227, N 5259.-P.680-685.