Nanocomposites and nanomaterials

‘Green’ synthesis and characterization of silver nanoparticles using Artemisia vulgaris ‘hairy’ root extracts as reducing agents
Kobylinska N.G.1, Kharchuk M.2, Duplij V.P.3, Matvieieva N.A.3
1 A.V. Dumansky Institute of Colloid Chemistry and Water Chemistry NAS of Ukraine, Ak. Vernadsky blvd. 42, Kyiv, 03142, Ukraine
2 Institute of Microbiology and Virology, NAS of Ukraine, 152 Ak . Zabolotny Str., Kyiv, 03143, Ukraine 
3 Institute of Cell Biology and Genetic Engineering, NAS of Ukraine, 148 Ak. Zabolotny Str., Kyiv, 03143, Ukraine
E-mail: joyna@ukr.net

Unique combination of antibacterial, optical, electrical, and catalytic properties of nanoparticles (NPs) stimulates research on study of design and functionalization for various applications. Progress is currently made in the technologies for the various syntheses of silver nanoparticles (AgNPs).
In this work, we compared the peculiarities of the formation of AgNPs using aqueous and ethanolic (70%) extracts from Artemisia vulgaris control and transgenic ("hairy") roots. The content of flavonoids and reducing activity of extracts from the control roots were lower than the corresponding parameters of "hairy" root extracts. The content of flavonoids and reducing power in all ethanolic (70%) extracts were greater than in aqueous one. 

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Fig. 1. TEM images of obtained AgNPs using aqueous (a,c) and ethanolic (b,d) extracts of control (c,d) and ‘hairy’ (a,b) roots.

The resulting colloidal solutions of all obtained AgNPs were stable during 6 month. AgNPs obtained using aqueous extracts were larger than those obtained by ethanolic extracts according TEM data (Fig. 1). NPs obtained by aqueous extracts from the control roots were polyhedral, while the hairy" root extracts produced oval shape NPs. Thus, the extracts properties depended on extraction solvents, root samples, etc. play a key role on the morphology and size of resulted AgNPs.