Nanocomposites and nanomaterials

Biocompatibility of γ-Fe2O3 nanoparticles with blood cells

Paryzhak S.Ya.1, Dumych T.I.1, Zasońska B.A.2, Svoboda J.2, Klyuchivska O.Yu.3, Skorokhyd N.R.3, Kozak Yu.S.3, Horák D.2 & Stoika R.S.1,3

1 Danylo Halytsky Lviv National Medical University, 69 Pekarska Str., Lviv 79010, Ukraine
E-mail: sola.paryzhak@gmail.com 
2 Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 162 06 Prague 6, Czech Republic
3 Institute of Cell Biology, National Academy of Sciences of Ukraine, 14/16 Drahomanov St., Lviv 79005, Ukraine

The importance of nanotechnologies for industry, power engineering, IT, medicine and other fields is constantly growing. Because of their small size, nanoparticles (NPs) penetrate into systemic circulation, quickly spread throughout the body and overcome biological barriers [1]. Thus, blood cells are the first contacts for the NPs entering the organism [2]. 
Our research was aimed at interaction between γ-Fe2O3 NPs (~9 nm) and their poly(N,N-dimethylacrylamide) and SiO2 coated derivatives and blood cells. By means of spectrophotometric measurements of released hemoglobin, it was found out that none of these three groups of NPs leads to erythrocytes damage. Incubation of NPs with isolated neutrophils resulted in slight granulocytes activation with the formation of neutrophil extracellular traps. Coating of γ-Fe2O3 with organic and inorganic shells decrease these effects. Besides, intravenous administration of NPs to laboratory animals did not manifest any toxic effect. 
Studies involving animals were conducted in accordance with the established experimental protocols and requirements of Ethics committee of Institute of Cell Biology NAS of Ukraine, protocol № 3/07102020. This work was supported by the Czech Science Foundation (No. 20-02177J).

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2.	Matus M.F., Vilos C., Cisterna B.A., Fuentes E., Palomo I. Nanotechnology and primary hemostasis: Differential effects of nanoparticles on platelet responses // Vascul Pharmacol.-2018.-101.-P. 1-8.