Nanochemistry and Nanobiotechnology

Promising nanobiotechnology to increase the shelf life of chicken eggs based on nanosilver preparation in hybrid carriers 
Zheltonozhskaya T.1, Shevchenko L.2, Permyakova N.1, Dovbnia Y.2,  Klepko V.1, Klymchuk D.3 
1 Department of Polymer Physics, Institute of Macromolecular Science of the NAS of Ukraine, 48 Kharkivske Shosse, 02160 Kyiv, Ukraine. 
E-mail: zheltonozhskaya@ukr.net
2 Department of Veterinary Hygiene, National University of Life and Environmental Sciences of Ukraine, 15 Heroyiv Oborony St., 03041 Kyiv, Ukraine.
3 Laboratory of Electron Microscopy, M.G. Kholodniy Institute of Botany of the NAS of Ukraine, 2 Tereshchenkivska St., 01601 Kyiv, Ukraine.

A simple and effective nanobiotechnology is proposed, aimed at reducing the endogenous and exogenous contamination of chicken eggs by opportunistic and pathogenic microflora in the processes of their formation and subsequent storage. The key element of this nanobiotechnology is a special preparation of nanosilver (dav=2.4±1.0 nm), stabilized in biocompatible and biodegradable hybrid carriers (Figure), which is orally administrated to laying hens with drinking water. 
	In this paper, various aspects of obtaining a nanosilver preparation in hybrid solutions and possible changes in its state under the influence of factors such as the pH of the solution, the concentration of nanoparticles, the presence of NaCl as in a "physiological solution", as well as visible light are considered. The high stability of this preparation in hybrid 	
Figure. TEM image of a nanosilver preparation in hydrophilic hybrid carriers		
carriers with respect to most of these factors was shown. 
Then the results of systematic studies of the biological impact of the developed nanosilver preparation on the clinical condition of laying hens, morphological and biochemical parameters of their blood, as well as morphological, microbiological and chemical parameters of chicken eggs are represented. A striking effect of the selective accumulation of nanosilver in the eggshell was found without deterioration in the condition, blood parameters and productivity of laying hens.