Nanobiotechnology for health-care

C60 fullerene effect on the degree of tremor oscillations of muscle contraction in chronic alcoholic rats

O. Motuziuk1, D. Nozdrenko2, K. Bogutska2, U. Ritter3, Yu. Prylutskyy2

1Lesya Ukrainka Volyn National University, ave. Voli, 13, 43025 Lutsk, Ukraine. 
E-mail: cmoplutsk@gmail.com
2Taras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01601 Kyiv, Ukraine. 
3Technical University of Ilmenau, Weimarer Str., 25, 98693 Ilmenau, Germany.

The effect of C60 fullerene aqueous solution (C60FAS) on the degree of tremor oscillations of the muscle soleus in chronic alcoholic rats with duration of alcoholism 6 months was studied. Experimental animals once a day received 40% ethyl alcohol at a dose of 2 ml/100 g weight. C60FAS at a dose of 1 mg/kg was mixed with an alcohol solution. Changes in the levels of tremor oscillations on stationary areas of muscle contraction were used as a marker to assess the level of miotic damage of the neuromuscular system, the severity of alcoholism in experimental animals and the therapeutic effect of C60FAS.
The tremor of maximal power responses with peaks from 11% (on the first contractions) to 38% (on the tenth contraction) of the level of stationary retention of the achieved strength position was observed during the formation of smooth tetanus in chronic alcoholic rats. The usage of C60FAS was shown to reduce the maximum amplitude of tremor oscillations by 18% on the first contractions and by 47% on the tenth contraction compared to the control. The positive dynamics of the therapeutic effect of C60FAS on the quality of accurate positioning of the studied muscle under its long-term stimulation was revealed. 
The established pronounced protective effect of C60FAS on the dynamics of muscle contraction in the development of alcoholic myopathy in animals opens up prospects for its practical use as a fundamentally new nanostructured medicine in the treatment of miotic pathologies.