Nanobiotechnology for health-care

Antimicrobial Properties of Nanocomposite Biomaterial for the Treatment of Purulent Wounds and Infected Ulcers
Sukhodub L.F.1, Kumeda M.O.1, Derevianko V.G. 1, Sukhodub L.B.1, 
1 Sumy State University. 2, Rymskogo-Korsakova st., 40007 Sumy,Ukraine. 
E-mail: l.sukhodub@med.sumdu.edu.ua

One of the important problems of modern surgery is the treatment of trophic ulcers of venous origin, which affect up to 3% of the population of industrialized countries. No less important is the issue of the ever-increasing resistance of microbial cells that contribute to the development of purulent-inflammatory processes to antibacterial drugs. All this encourages the search for new methods and materials for the treatment of purulent-inflammatory diseases without the use of antibiotics or with restrictions on their use, which determines the relevance of this problem. The proposed antiseptic apatite-polymer scaffold is a macroporous three-dimensional network formed by the polyelectrolyte interaction between macromolecules of polymers of natural origin - sodium alginate (Alg) and chitosan (CS), in the pores of which immobilized particles of calcium deficiency hydroxyapatite. The surface chitosan layer of the scaffold is additionally doped with divalent metal ions Ca2+, Zn2+, Mg2+, Cu2+, and Fe2+, which form chelate complexes with CS and have enhanced antimicrobial action [1]. To assess the antiseptic ability of experimental samples against Gram-positive S. Aureus ATCC 25923 and Gram-negative E. coli ATCC 25922 microorganisms, the calculation of the integrated indicator (A) of their antimicrobial activity was performed [2]. The applied vector theory allowed to present A as a vector in n-dimensional space with coordinates in the form of a zone of growth inhibition for each test microorganism. The results showed that the value of A for samples containing ions Ca2+, Zn2+, Mg2+, Cu2+, and Fe2+ is 1.85, 2.08, 1.1, 1.9, 1.65, respectively. Thus, according to the guidelines with an efficiency range of more than 1.5, all samples, except Mg2+ - containing, are those that show average antimicrobial activity, and Zn2+ - close to Chlorhexidine (2.07).

1.	L.B. Sukhodub, Metal Ions Doped Chitosan Nanoparticles // JNEP.-2014.- 6(4).- 04034(6pp).
2.	MES of Ukraine, NAMSU Methods of calculating integrated indicators of antimicrobial activity of antibacterial drugs // Methodical recommendations. (79.15 / 02.16).-P. 29.