Nanobiotechnology for health-care

Two kinds of luminescence centers in low-temperature phosphorescence spectra of IPNV RNA
Losytskyy M.Y.1, Kravchenko V.M.1, Rud Y.P.1,2, Buchatskyi L.P.1,2, Zaloilo O.V.1,2, Yashchuk V.M.1
1 Taras Shevchenko National University of Kyiv, Volodymyrs’ka Str., 64/13, Kyiv, 01601, Ukraine. 
E-mail: mlosytskyy@gmail.com
2 Institute of Fisheries of NAAS of Ukraine, Obukhivska Str., 135, Kyiv, 03164, Ukraine.

Viral infections are among the most important challenges for the modern world; thus the study of the properties of these nanosized biological objects is crucial for understanding the ways of fighting this challenge. Particularly, the electronic processes in viral nucleic acids and proteins determine the efficiency of the deactivation of viruses by electromagnetic irradiation. To understand these processes, spectral properties of viruses and their components (nucleic acids and proteins) should be studied. RNA viruses of fish are efficient models for the study of viral pathogenesis and its treatment. Important representative of this class of viruses is the infectious pancreatic necrosis virus (IPNV) which causes a highly contagious disease of salmonid fish. Earlier, spectral properties of IPNV virions, RNA and major capsid proteins were studied by us [1].
Here, we have studied the low-temperature luminescence properties of RNA molecules of IPNV in more details. The phosphorescence spectra of RNA reveal the presence of two kinds of luminescence centers, each of them manifested as structured phosphorescence spectrum with maxima near 402, 430 and 460 nm for “center I”, and 415, 444 and 477 nm for “center II”. The both centers have shifted excitation spectra, “center II” is also characterized by longer phosphorescence decay time compared to “center I”. The nature of these centers and electronic processes in the RNA of IPNV were discussed. 
The work is supported by the Ministry of Education and Science of Ukraine (Grant 21BF051-03)

1.	Kravchenko V. M., Rud Y. P., Buchatski L. P., Stepanenko Y. Y., Gryn D. V., Yashchuk V. M. Spectroscopic Studies of Infectious Pancreatic Necrosis Virus, Its Major Capsid Protein, and RNA // Ukr J Phys.-2019.-64, N 2.-P.120-125.