Nanobiotechnology and chemical technology

Effect of Fe(II, III) and Co(II) salts on morphology and structure of “green” synthesized magnetic nanoparticles

Matvieieva N. 1, Kobylinska N. 2, Klymchuk D.3, Khainakova O.4, Kuchuk M.1

1 Institute of Cell Biology and Genetic Engineering, NAS of Ukraine, 148 Zabolotnogo Str, Kyiv, 03143, Ukraine E-mail:joyna@ukr.net 
2Dumansky institute of colloidal chemistry and water chemistry, NAS of Ukraine 42 Vernadsky blv., Kyiv, 03142, Ukraine 
3M.G. Kholodny Institute of Botany, NAS of Ukraine, 2 Tereshchenkivska Str, Kyiv, 02000, Ukraine 
4University of Oviedo, 8 Julián Claveria Av.,Oviedo, 33006, Spain.

     Iron oxide magnetic nanoparticles (MNPs) have been used due to their unique properties, as well as their biocompatibility and low toxicity. New approach for non-chemical synthesis of MNPs is based on the using of nontoxic plant extracts with high reducing activity. In this work different Fe(II, III) and Co(II) salts combinations were used for MNPs synthesis initiated by the addition of the extracts of Artemisia annua L ‘hairy’ roots. We prepared the series of MNPs on the base of mixtures of salts (FeCl2, FeCl3, Fe(NO3)3, FeSO4, CoCl2) in different combinations and examined their morphology and structural properties. Effect of the reaction mixture content on morphology and size of MNPs was controlled by TEM.

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Fig. 1. TEM images of MNPs by using of salts: a – FeCl3+FeSO4, b –FeCl3+FeCl2+FeSO4+CoCl2 mixtures.

(a)

‘Hairy’ root extract adding successfully resulted in nanoparticles formation. The MNPs were synthesized using only divalent salts of metal ions (FeCl2+ FeSO4) with obtaining of large size dispersed MNPs. The TEM images demonstrated quite round and irregular shape, polydispersion and high size distribution of NPs obtained by mixture of metal ions of different valents (Fig. 1). The reaction mixture for synthesis MNPs from salt solution by the adding of CoCl2 resulted in the formation of uniform and smaller (10.5-16.0 nm) NPs than the one obtained using mono valence metal salt reaction mixture.

(d)