Nanobiotechnology and chemical technology

Effect of Fe (II, III) and Co(II) salts on morphology and structure of “green” synthesized magnetic nanoparticles

Kobylinska N.1, Klymchuk D.2, Khainakova O.3, Matvieieva N.4

1A.V. Dumansky Institute of Colloid Chemistry and Water Chemistry NAS of Ukraine, Ak. Vernadsky blv. 42, Kyiv, 03142, Ukraine
2M.G. Kholodny Institute of Botany, NAS of Ukraine, 2 Tereshchenkivska Str, Kyiv, 02000, Ukraine
3University of Oviedo, 8 Julián Claveria Av., Oviedo, 33006, Spain 
4Institute of Cell Biology and Genetic Engineering, NAS of Ukraine, 148 Zabolotnogo Str, Kyiv, 03143, Ukraine, email: joyna@ukr.net

Iron oxide magnetic nanoparticles (MNPs) have been used due to their unique properties, as well as their biocompatibility and low toxicity. New approach for non-chemical synthesis of MNPs is based on the using of nontoxic plant extracts with high flavonoids content. In this work different Fe(II, III) and Co(II) salts combinations were used for MNPs synthesis initiated by the addition of the extracts of Artemisia annua L ‘hairy’ roots. The series of MNPs on the base of mixtures of salts (FeCl2, FeCl3, Fe(NO3)3, FeSO4, CoCl2) in different combinations were prepared and their morphology and structural properties were examined. Effect of the reaction mixture content on morphology and size of MNPs was controlled by TEM.
‘Hairy’ root extract adding to inorganic salts successfully resulted in MNPs formation. The MNPs were synthesized using only divalent salts of metal ions (FeCl2+FeSO4) with obtaining of large size dispersed MNPs. The TEM images demonstrated quite round and irregular shape, polydispersion and high size distribution of MNPs obtained by mixture of only Fe(II, III) ions. The reaction mixture for synthesis MNPs from Fe(II, III) solutions by the adding of CoCl2 resulted in the formation of uniform and smaller (10.5-16.0 nm) MNPs than the one obtained using mono valence metal salt reaction mixture (Fig. 1). The peaks of powder XRD patterns of the obtained MNPs indicate the formation of magnetite with a cubic inverse spinel structure with several impurities.

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Fig. 1. TEM images of MNPs by using of salts: a – FeCl2+FeSO4, b –FeCl3+FeCl2+FeSO4+CoCl2 mixtures.

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