Nanochemistry and biotechnology

Potentiometric enzyme biosensor modified with gold nanoparticles
Shkotova L.V.1, Zinchenko O.A.1, Arkhypova V.M.1, Dzyadevych S.V.1,2
1 Department of Biomolecular Electronics, Institute of Molecular Biology and Genetics, NAS of Ukraine, 150, Zabolotnogo Str., Kyiv-03143, Ukraine.
E-mail: luda_shkotova@yahoo.com
2 Institute of High Technologies, Taras Shevchenko Kyiv National University,
2 Hlushkova Ave., Kyiv-03022, Ukraine.

The ever-increasing number of necessary clinical tests in medical diagnostics requires highly sensitive, selective, rapid and economic methods of analysis. The decisive factors of the development of laboratory diagnostics are the elaboration and implementation of new technologies to meet the main requirements to modern laboratory equipment - reliability, efficiency and ease-of-use.
A highly sensitive, selective potentiometric biosensor based on immobilized butyrylcholinesterase was developed; its laboratory prototype has been created. The enzyme served as a sensitive element and pH-sensitive field-effect transistors (FETs) were used as the transducers of a biochemical signal into electrical signal.
Because butyrylcholinesterase has been already used to develop the sensors for determination of heavy metals, organophosphorus/carbamate pesticides and glycoalkaloids, the present study has been focused on the investigation and adjustment of specificity and sensitivity of the potentiometric biosensor exactly for the case of using gold nanoparticles.
The composition of a butyrylcholinesterase-based bioselective membrane with gold nanoparticles was optimized. Optimal conditions of the biosensor functioning, such as pH and ionic strength of the working buffer solution, were selected. The signal reproducibility of the developed biosensor and its storage stability were studied.
The work was carried out thanks to financial support from the National Research Fund of Ukraine in the framework of the competition of projects for research and development "Support of research of leading and young scientists" (project 2020.02/0097).