Application nanosorbents with antimicrobial and anaesthetic properties
Gaidai A., Furtat I., Vakuliuk P., Golub A.
National University of Kyiv-Mohyla Academy, 2 Skovoroda Str., Kyiv, 04070, Ukraine. E-mail: alina.gaidai@ukma.edu.ua
Solving the problem of finding the latest effective drugs to accelerate the healing of complicated wounds requires the creation of application sorption materials that simultaneously have proteolytic, antibacterial and anaesthetic properties. Due to the low initial toxicity of nanocomposite materials and the acquisition of other activities, the latter is considered promising for use in intensive care of wound surfaces and other injuries. Thus, the combination of highly dispersed silica with antiseptics and anaesthetics allows obtaining anti-inflammatory and wound-healing application materials for the treatment of wound infection.
The work aimed to obtain application sorbents based on highly dispersed silica with ornidazole, urotropin and lidocaine immobilized on the surface, and to study their antibacterial properties for further use as effective innovative tools in complex therapy of complicated wounds, ulcers and burns. As in our previous works, nanocomposite materials were obtained by impregnating a silica matrix with a mixture of active components. Determination of biocidal activity was carried out by seeding cell suspensions of S. aureus strains ATCC 25923 and E. coli ATCC 25922 after their exposure to the created nanocomposites for 2 and 24 hours. Then, cell survival indices (SI) were calculated, ie the percentage of bacterial cells that survived after contact with these nanocomposites (Dolange et al, 2018).
Studies of the stability of the obtained composites and the rate of release of ornidazole, urotropin, lidocaine, confirmed the prolongation of desorption of active substances into aqueous solution from the surface of nanocomposites obtained based on silica matrix.
A study of the bactericidal effect of nanocomposites impregnated with ornidazole, urotropin and lidocaine at two different time intervals showed that exposure for 2 hours did not provide a significant bactericidal effect. Instead, it was found that for the effective manifestation of nanocomposite activity, the interaction time with test cultures should be at least 24 hours. Thus, under the action of the nanocomposite "ornidazole-urotropin" on S. aureus cells, the survival index was 7.4%, and under the influence of the nanocomposite "ornidazole-urotropin-lidocaine" - SI = 2.5%, compared with SI = 52.1% and SI = 30.4% for 2 hours of contact, respectively. In the case of E. coli cells under similar conditions, an almost complete biocidal effect was recorded, because under the action of these nanocomposites, SI values were less than 0.1%. Based on the obtained data, it can be stated that the biocidal efficiency of the nanocomposite "ornidazole-urotropin-lidocaine" is higher compared to the nanocomposite "ornidazole-urotropin". In our opinion, the detected effect is due to the introduction of lidocaine, which is consistent with the data of other authors (Kesici et al, 2019), according to which the combined use of lidocaine with other pharmacopoeial drugs may be clinically relevant in the prevention and treatment of wound healing.