Nanobiotechnology for health-care

Effect of copper nanoparticles and copper nanoparticles nanoemulsion on the healing of burn wounds

Shtapenko O. V.1, Syrvatka V. Ya.1, Horbay R.2, Slyvchuk O. Yu.1

1 Institute of Animal Biology National Academy of Agrarian Science of Ukraine  V. Stusa, 38, Lviv-79034, Ukraine. 
E-mail: shtapenko31@gmail.com
2Cancer Therapeutics Program, Ottawa Hospital Research Institute, Ottawa, Canada

The discovery and production of antibiotics was a major step forward in pathogen elimination, and one of the greatest achievements in medicine. However, excessive and inappropriate use of antibiotics leads to an emerging need to search for new effective broad-spectrum antibacterial agents [1]. Copper nanoparticles have shown great potential as antimicrobial agents with low levels of toxicity [2]. Besides the improved wound healing effect of nanoparticles can be potentially achieved by incorporating a particle into nanoemulsion formulation [3]. In this work, the wound healing properties of Copper Nanoparticles (CuNPs) and Copper Nanoparticles Nanoemulsion(CuNPs-NE) an experimental model of second-degree thermal burns in male Wistar rats has been studied.
It was shown that CuNPs and CuNPs-NE significantly speeded up the process of wound healing and decreased wound area in comparison to control.  Significant wound healing was observed after CuNPs-NE treatment on day 6, 8, 10, 12 compared to the control group (P<0.05-0.01) and to CuNPs colloid treated group (P<0.05). Furthermore, histological analysis confirmed better wound healing effect after CuNPs-NE treatment.  So, encapsulating of nanoparticles in nanoemulsion can improve the therapeutic potential and promote wound healing.

1.	Baptista P.V., McCusker M.P., Carvalho A., Ferreire D.A., Mohan N.M., Martins M., et al. Nano-strategies to fight multidrug resistant bacteria —“A Battle of the Titans”// Front Microbiol. – 2018. -9. –P. 1441.
2.	Champagne V.K., Helfritch D.J. A demonstration of the antimicrobial effectiveness of various copper surfaces //  J Biol Eng. – 2013. -7. – P 8.
3.	Hajialyani M., Tewari D., Sobarzo-Sanchez., Nabavi SM, et al. Natural product-based nanomedicines for wound healing purposes: therapeutic targets and drug delivery systems // Int J Nanomedicine. – 2018. -13. –P. 5023–5043.