Nanocomposites and nanomaterials

The interaction of silver-enriched clinoptilolite with mammalian immunocompetent cells

Paryzhak S.Ya.1, Dumych T.I.1, Klyuchivska O.Yu.2, Manko N.O.2, Gryshchouk G.V.3, Vasylechko V.O.3, Stoika R.S.2

1 Danylo Halytsky Lviv National Medical University, 69 Pekarska Str., Lviv 79010, Ukraine
E-mail: sola.paryzhak@gmail.com 
2 Institute of Cell Biology, National Academy of Sciences of Ukraine, 14/16 Drahomanov St., Lviv 79005, Ukraine
3 Ivan Franko National University of Lviv, 6 Kyryla and Mefodiya Str., Lviv 79005, Ukraine

Owing to their unique chemical and physical properties, zeolites are widely used in agriculture, industry, medicine, cosmetology and other fields. Natural zeolite – clinoptilolite, is permitted to be utilized for medical and veterinary applications [1]. In spite of the fact that Ag-doped clinoptilolite possesses toxic impact on tumors and pseudo-normal mammalian cells in vitro [2], its influence on immune cells, as well as on the development of immune response has still to be thoroughly studied.
Our research was aimed at interaction between silver-enriched natural clinoptilolite (from the deposit near the Sokirnytsia village in the Transcarpathian region of Ukraine) and mammalian immune cells. We found out that the silver-enriched clinoptilolite influences neutrophil granulocytes with their further activation in vivo. It also gives rise to humoral immune response in vivo. The investigated samples manifested moderate immunogenicity and proved to be safe for in vivo application. All the works including housing and care, method of euthanasia were conducted in accordance with the established experimental protocols and requirements of Ethics committee of Institute of Cell Biology NAS of Ukraine, protocol № 3/07102020. This work was supported by the grant from the Ministry of Education and Science of Ukraine KhA-87F, 2019-2021.

1.	Kraljević Pavelić S, Simović Medica J, Gumbarević D, Filošević A, Pržulj N, Pavelić K. Critical Review on Zeolite Clinoptilolite Safety and Medical Applications in vivo // Front Pharmacol.-2018.-9.-P. 1350. 
2.	Vasylechko V., Klyuchivska O., Manko N., Gryshchouk G., Kalychak Ya., Zhmurko I., Stoika R. Novel nanocomposite materials of silver-exchanged clinoptilolite with preconcentration of Ag(NH3)2+ in water possess enhanced anticancer action // Appl Nanosci.-2020.-10, N 12.-P. 4869-4878.