Nanochemistry and biotechnology
Influence of AG+ ions in DNA spectral properties
Gryn D.V.1, Yashchuk V.M.1
1 Taras Shevchenko National University of Kyiv, Physics faculty,
Volodymyrs’ka str. 64/13, Kyiv, 01601, Ukraine. 
E-mail: d.gryn@ukr.net

DNA is vitally important macromolecule and often used in various fields of biochemistry, biology and medicine.
In many experiments the DNA fluo- and phosphorescence emission is observed and investigated. 
It is well known the property of Ag+ ions to accumulating on the DNA, from sepatate ions in solution to some small nano-clusters near DNA, and then to clusters and nanoparticles. This is often used in controlled nanoparticles grows on DNA used as a template.
In this work we analyze the influence of presence of Ag+ ions on the DNA spectral properties.
Water solutions of DNA, and DNA-Ag samples of different DNA:Ag ratios were investigated at room and nitrogen (77K) temperatures.
The UV-vis spectra as well as fluorescence and phosphorescence spectra of DNA in presence of different quantity of Ag were compared to pure DNA spectra.
Conclusions have been made about the relationship between shape of the spectra, Ag+ quantity and macromolecule structure. 

1.	Grebinyk, A., Yashchuk, V., Bashmakova, N. et al. A new triple system DNA-Nanosilver-Berberine for cancer therapy // Applied Nanoscience.-2019.-9,.-P. 945–956.