Nanobiotechnology for health-care
Cold and menthol receptor activation TRPM8 improves post-traumatic recovery of rat muscle soleus in C60 fullerene therapy 

Matvienko T. Yu. 1, Nozdrenko D. M. 1, Bogutska K. I. 1, Prylutskyy Y.I. 1, Zholos A. V. 1

1ESC”Institute of Biology and Medicine”,Taras Shevchenko National University of Kyiv, Volodymyrska Str. 64, Kyiv 01601, Ukraine; 
E-mail:tamatvienko@gmail.com

Mechanical damage is the most common type of skeletal muscle injury, which is divided into the following types: stretching, bruising and tearing. The first two of them do not represent a serious threat of loss of control over muscle function. However, rupture of muscle tissue, especially in its severe forms, without appropriate treatment can manifest itself as a fatal pathology leading to complete or partial disability or even death of the patient [1]. 
A common feature of all muscle injuries is the ability to recover through a process called inflammatory reaction. This reaction is an adaptive function of the body and is aimed at destroying the agent that caused the process and restoring damaged tissue [2]. 
The biomechanical parameters of muscle soleus of rats during the first 3 days of development of the posttraumatic syndrome caused by destruction of muscle cells by means of mechanical compression were studied. As therapeutic agents, C60 fullerenes as powerful antioxidants [3] as well as the selective agonist of TRPM8 cold receptors – menthol were used. Injections of water-soluble pristine C60 fullerene (at concentration of 1 mg/kg) into the damaged muscle improved its contractile function by 25-30%. Application of combined injections of C60 fullerene and menthol (at concentration of 1 mg/kg) improved this indicator by an extra 17-19%, while stabilizing the decrease in muscle strength observed throughout all experiments. The revealed synergy effect of above therapeutics on posttraumatic process of restitution of skeletal muscle function opens up new prospects for their clinical application. 

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3.	Gonchar O.O., Maznychenko A.V., Bulgakova N.V. et al. C60 fullerene prevents restraint stress-induced oxidative disorders in rat tissues: possible involvement of the Nrf2/ARE-antioxidant pathway // Oxid. Med. Cell. Longev. -2018.- Article ID 2518676.