Nanobiotechnology for health-care

Influence of Synthesis Technique on the Bioactivity of Chitosan-Hydroxyapatite Nanocomposites 

Sukhodub L.B., Sukhodub L.F., Kumeda M.A.
Sumy State University, Rimsky-Korsakov,2, Sumy-40007, Ukraine.
E-mail: l.sukhodub@gmail.com

The work is devoted to the biomimetic creation and research of the biomaterials based on hydroxyapatite and chitosan for bone tissue repair. The biocompatibility of chitosan (it consists only of carbohydrates, has no protein‵s compounds, which is why it is not alien to the body and is not rejected), its biodegradation (gradual and slow decomposition under the influence of lysozyme and lysosomal phagocyte enzymes) in combination with antibacterial properties make it an ideal material for the manufacture prostheses of bones, teeth, vessels, coatings for the metal parts of prostheses. Chitosan-based implants promote the proliferation and maturation of osteoblasts, enhance protein synthesis and bone mineralization.
Our previous in vivo studies have proved the optimality of the beads-shaped composite materials, based on hydroxyapatite (HA) and chitosan (CS), to fill bone defects with a complex geometry. The techniques described in our resent research [1,2] suggest the formation of beads as a result of mechanical mixing of HA and polymers (alginate, chitosan), which was reflected in the properties of composite materials.
The purpose of this work was to simulate the process of synchronous calcium phosphate crystallites and the chitosan matrix formation during the synthesis of a beads-shaped composite material containing a drug. Previous in vitro studies have demonstrated the high surface bioactivity of the synthesized material and its antimicrobial properties. 

1.	Sukhodub L.B., Sukhodub L.F., Kumeda M.O., et al. C60 fullerene loaded hydroxyapatite-chitosan beads as a promising system for prolonged drug release // CarboPol.-2019.-223,- 115067.
2.	Sukhodub L.B., Sukhodub L.F., Litsis O., Prylutskyy Y.I. Synthesis and characterization of hydroxyapatite-alginate nanostructured composites for the controlled drug release // Mat Chem and Phys.-2018.-217, -P. 228-234.