Nanocomposites and nanomaterials

Preparation and Characterization of Liposomal Murrayafoline A
Dubatouka K.I.1, Agabekov V.E.1, Quoc Toan Tran2, The Dan Pham2, Quoc Long Pham2
1 Institute of Chemistry of New Materials NASB, Skoryny str., 36, Minsk-220041, Belarus, E-mail: d_katerina@tut.by
2 Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam

Murrayafoline A (MuA), isolated from Murraya koenigii in Vietnam, is a carbazole derivative that shows biological activity as cytotoxic agent [1, 2]. Liposomal form of MuA (Lip-MuA), obtained using combination of the thin film hydration and sonication methods, can be used to reduce the drug toxicity, improve its bioavailability and create the drug delivery system. 
Liposomes were prepared from the mixtures of egg phosphatidylcholine (PC), cholesterol (Ch) and MuA in chloroform. The mean diameter (D) and zeta potential (ZP), measured using dynamic light scattering, were in range from 190 to 520 nm and -44.0 to -32.0 mV for different formulations respectively. 
Formulations	D, nm	ZP, mV	
PC,Ch,MuA 5%	222±23	-44.8±0.6	
PC,MuA 5%	300±44	-37.3±2.0	
PC,Ch,MuA 10%	220±28	-43.2±0.3	
PC,MuA 10%	192±21	-31.8±2.3	
PC,Ch,MuA 20%	254±32	-43.8±0.6	
PC,MuA 20%	520±160	-37.6±3.4	
Table 1. Characteristics of Lip-MuA. 

Liposomes from PC, Ch and 5–10% (wt.) of MuA had less size as well as zeta potential which indicates its higher stability in comparison with Lip-MuA (20%) and cholesterol-free formulations. 
The stability studies, performed at 200C for 7 days, were shown no significant difference in size and zeta potential for Lip-MuA formulations.
It was obtained and characterized stable negatively charged liposomal Murrayafoline A (5 - 10% wt.) that can be used in biomedicine.
This research is funded by VAST under grant number QTBY01.03 / 20-21 and BRFFR under grant number X20V-001.

1.	L.Anh, N.Thi, H.Ly et.al. Synthesis and cytotoxic activity evaluation of novel derivatives of murrayafoline A// J. of Science and Technology.-2016.- 54 (2C).-P. 502-508.
2.	Itoigawa M., Kashiwada Y., et al. - Antitumor agent. 203. Carbazole alkaloids murrayaquinone A and related synthetic carbazolequinones as cytotoxic agents// J. Nat.Prod. -2000.-63.- P.893-897.