Nanobiotechnology for health-care

С60 fullerene nanoparticles as modulators of mechanokinetic changes in skeletal muscle function under acute inflammation
V. Bilobrov1, D.O. Zavodovskyi1, T.Yu. Matvienko2, N.V. Bulgakova1,
Yu.I. Prylutskyy2 

1Bogomoletz Institute of Physiology, 01601 Kyiv, Ukraine 
E-mail: cyper29695@gmail.com
2Taras Shevchenko National University of Kyiv, ESC “Institute of Biology and Medicine”, 01033 Kyiv, Ukraine

Studies for the past ten years show the prospect of C60 fullerenes as therapeutic agents for a wide range of medical pathologies. Inflammation of skeletal muscles occupies a special place among them, since this pathology can occur both as independently and as a result of complication after surgical interventions.
The acute formalin-induced model in musculus gastrocnemius of adult outbred male Wistar rats was studied. Studies have been aimed at identifying the therapeutic effect of water-soluble C60 fullerenes. Considering the fact that the integrative indicator of skeletal muscle condition is the fulfillment of its main function – contraction, to reveal the therapeutic effect of C60 fullerene nanoparticles we have recorded changes in mechanokinetic parameters of contraction in the first three hours after initiation of acute inflammation. Muscle activity was recorded under different modes in tetanic and pre-tetanic contractions with using servo-controlled stretching and relaxing electrical stimulation of the muscle.
It was found that the signified therapeutic effects of water-soluble C60 fullerenes occur at all applied frequencies (2, 14 and 50 Hz) of electrical stimulation of the nervus ischiadicus. They can be divided into two main categories: delay onset of mechanokinetic pathological changes (from 20 min to an hour depending on the contraction mode) and level reducing of pathological changes at the stage of their development, which were most significant (over 40%) in the pre-tetanic contraction mode.