Nanobiotechnology for health-care

XPS Investigation of Interaction the Virus Hybrid Structure with Au Nanoparticles
Kurgan N.A., Karbovska L.I., Karbivskyy V.L.
G.V. Kurdymov Institute for Metal Physics of the NAS of Ukraine,
03680, blvd. Vernadsky, 36, Kiev, Ukraine 
E-mail: natalkakurgan@gmail.com  

A key point in the creation of nanobiosensors is the selection and diagnosis of optical and electrical properties of an immobilizing carrier. To solve this problem, the most promising is the use of tobacco mosaic virus (TMV) [1].

Fig. 1. X-ray photoelectron spectra of Au 4f for both Au nanoparticles and Au-TMV.

A study carried out of the physicochemical properties and interaction of the hybrid structure based on the tobacco mosaic virus and gold nanoparticles (Au-TMV) obtained by X-ray photoelectron spectroscopy. Nanostructure had sizes about 50 nm in diameter and 150-400 nanometers in length. The size of the gold nanoparticles was about 20 nm.
It is established that their interaction with the substrate is characterized by С–N bond, while the bond N-C=O is characterized by binding virions with each other. the electronic 4f core levels of aurum demonstrate shift towards lower binding energy at Au-TMV formation (fig. 1). Decrease in the binding energy of the core electrons of Au 4f during the transition from gold nanoparticles to Au‑TMV complexes indicates an increase in the electron density on gold atoms and a increase of the ionic component in the total chemical bond balance.

1.	Hamdy ME, Del Carlo M, Hussein HA, Salah TA, El-Deeb AH, Emara MM, Pezzoni G, Compagnone D (2018) Development of gold nanoparticles biosensor for ultrasensitive diagnosis of foot and mouth disease virus. J Nanobiotechnology 16:48.